NF EN ISO 21872-1
Microbiology of the food chain - Horizontal method for the determination of Vibrio spp - Part 1 : detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.ISO 21872-1:2017 is applicable to the following:- products intended for human consumption and the feeding of animals;- environmental samples in the area of food production and food handling.
ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
ISO 21872-1:2017 is applicable to the following:
- products intended for human consumption and the feeding of animals;
- environmental samples in the area of food production and food handling.
Le présent document spécifie une méthode horizontale de recherche des deux principales espèces pathogènes de Vibrio provoquant des maladies intestinales chez l'homme : les V. parahaemolyticus et les V. cholerae.
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1 Domaine d'application
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2 Références normatives
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3 Termes et définitions
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4 Principes
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5 Milieux de culture et réactifs
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6 Matériel et consommables
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7 Échantillonnage
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8 Préparation de l'échantillon pour essai
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9 Mode opératoire (voir Figure A.1)
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10 Expression des résultats
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11 Caractéristiques de performance de la méthode
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12 Rapport d'essai
- Annexe A Schéma du mode opératoire
- Annexe B Composition et préparation des milieux de culture et des réactifs
- Annexe C PCR classique pour la recherche de Vibrio parahaemolyticus, des gènes de l'hémolysine thermostable directe, TDH (tdh) , et de l'hémolysine apparentée à la TDH (trh) , de Vibrio cholerae et de Vibrio vulnificus
- Annexe D PCR en temps réel pour la recherche de Vibrio parahaemolyticus, du gène de l'hémolysine thermostable directe, TDH (tdh) , et de Vibrio vulnificus
- Annexe E Résultats d'une étude interlaboratoires
- Bibliographie
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