NF EN ISO 22119

NF EN ISO 22119

October 2011
Standard Current

Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions

ISO 22119:2010 defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). ISO 22119:2010 specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.The minimum requirements laid down in ISO 22119:2010 provide the basis for comparable and reproducible results within individual and between different laboratories.ISO 22119:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.

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Main informations

Collections

National standards and national normative documents

Publication date

October 2011

Number of pages

22 p.

Reference

NF EN ISO 22119

ICS Codes

07.100.30   Food microbiology

Classification index

V08-204

Print number

1 - 28/09/2011

International kinship

ISO 22119:2011

European kinship

EN ISO 22119:2011
Sumary
Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions

ISO 22119:2010 defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). ISO 22119:2010 specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.

The minimum requirements laid down in ISO 22119:2010 provide the basis for comparable and reproducible results within individual and between different laboratories.

ISO 22119:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.

Table of contents
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principe
    5
  • 4.1 Généralités
    5
  • 4.2 Sondes pour PCR en temps réel
    5
  • 5 Exigences générales relatives aux laboratoires
    6
  • 6 Réactifs et matériaux
    6
  • 7 Appareillage
    7
  • 8 Échantillon pour laboratoire
    8
  • 9 Mode opératoire
    8
  • 9.1 Préparation de l'échantillon
    8
  • 9.2 Amplification
    8
  • 9.3 Témoins
    9
  • 9.4 Analyse des données de fluorescence
    9
  • 10 Évaluation et documentation
    11
  • Bibliographie
    12
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