NF EN ISO 22718

NF EN ISO 22718

September 2009
Standard Cancelled

Cosmetics - Microbiology - Detection of Staphylococcus aureus

ISO 22718:2006 gives general guidelines for the detection and identification of the specified micro-organism Staphylococcus aureus in cosmetic products. Micro-organisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which ISO 22718:2006 is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. The method described in ISO 22718:2006 is based on the detection of Staphylococcus aureus in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required. Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise validated.

View the extract
Main informations

Collections

National standards and national normative documents

Publication date

September 2009

Number of pages

23 p.

Reference

NF EN ISO 22718

ICS Codes

07.100.40   Cosmetics microbiology

Classification index

T75-605

Print number

1 - 01/09/2009

International kinship

European kinship

EN ISO 22718:2009
Sumary
Cosmetics - Microbiology - Detection of Staphylococcus aureus

ISO 22718:2006 gives general guidelines for the detection and identification of the specified micro-organism Staphylococcus aureus in cosmetic products. Micro-organisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which ISO 22718:2006 is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.

The method described in ISO 22718:2006 is based on the detection of Staphylococcus aureus in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required.

Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise validated.

Replaced standards (1)
NF ISO 22718
September 2006
Standard Cancelled
Cosmetics - Microbiology - Detection of Staphylococcus aureus

<p>ISO 22718:2006 gives general guidelines for the detection and identification of the specified micro-organism <i>Staphylococcus aureus </i>in cosmetic products. Micro-organisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.</p> <p>In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which ISO 22718:2006 is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.</p> <p>The method described in ISO 22718:2006 is based on the detection of <i>Staphylococcus aureus</i> in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required.</p> <p>Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise validated.</p>

Standard replaced by (1)
NF EN ISO 22718
February 2016
Standard Current
Cosmetics - Microbiology - Detection of Staphylococcus aureus

<p>ISO 22718:2015 gives general guidelines for the detection and identification of the specified microorganism Staphylococcus aureus in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.</p> <p>In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.</p> <p>The method described in this International Standard is based on the detection of Staphylococcus aureus in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required.</p> <p>NOTE For the detection of Staphylococcus aureus, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).</p> <p>Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.</p>

Table of contents
View the extract
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principe
    2
  • 5 Diluants et milieux de culture
    2
  • 5.1 Généralités
    2
  • 5.2 Diluant pour la suspension bactérienne (solution de tryptone et de chlorure de sodium)
    3
  • 5.3 Milieux de culture
    3
  • 6 Appareillage et verrerie
    6
  • 7 Souches de microorganismes
    6
  • 8 Manipulation des produits cosmétiques et des échantillons de laboratoire
    7
  • 9 Mode opératoire
    7
  • 9.1 Recommandations générales
    7
  • 9.2 Préparation de la suspension initiale dans le milieu liquide d'enrichissement
    7
  • 9.3 Incubation du milieu liquide d'enrichissement ensemencé
    8
  • 9.4 Détection et identification de Staphylococcus aureus
    8
  • 10 Expression des résultats (détection de Staphylococcus aureus)
    9
  • 11 Neutralisation des propriétés antimicrobiennes du produit
    9
  • 11.1 Généralités
    9
  • 11.2 Préparation de l'inoculum
    9
  • 11.3 Validation de la méthode de recherche
    9
  • 12 Rapport d'essai
    10
  • Annexe A (informative) Autres milieux
    11
  • Annexe B (informative) Neutralisants de l'activité antimicrobienne des conservateurs et liquides de rinçage
    14
  • Bibliographie
    15
Need to identify, monitor and decipher standards?

COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.

Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!

Request your free, no-obligation live demo

I discover COBAZ