NF EN ISO 30024

NF EN ISO 30024

October 2009
Standard Cancelled

Animal feeding stuffs - Determination of phytase activity

ISO 30024:2009 specifies the determination of phytase activity in feed samples.The method does not distinguish between phytase added as a feed additive and endogenous phytase already present in the feed materials.The method cannot be used to evaluate or compare the in vivo efficacy of the phytase product. It is not a predictive method of the in vivo efficacy of phytases present on the market as they can develop different in vivo efficacy per unit of activity.The method is suitable and validated exclusively for the determination of phytase activity and exclusively in complete feeds.

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Collections

National standards and national normative documents

Publication date

October 2009

Number of pages

21 p.

Reference

NF EN ISO 30024

ICS Codes

65.120   Animal feeding stuffs

Classification index

V18-224

Print number

1 - 02/10/2009

International kinship

ISO 30024:2009

European kinship

EN ISO 30024:2009
Sumary
Animal feeding stuffs - Determination of phytase activity

ISO 30024:2009 specifies the determination of phytase activity in feed samples.

The method does not distinguish between phytase added as a feed additive and endogenous phytase already present in the feed materials.

The method cannot be used to evaluate or compare the in vivo efficacy of the phytase product. It is not a predictive method of the in vivo efficacy of phytases present on the market as they can develop different in vivo efficacy per unit of activity.

The method is suitable and validated exclusively for the determination of phytase activity and exclusively in complete feeds.

Table of contents
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Termes et définitions
    1
  • 3 Principe
    1
  • 4 Réactifs
    2
  • 5 Appareillage
    3
  • 6 Échantillonnage
    4
  • 7 Préparation de l'échantillon
    4
  • 8 Mode opératoire
    4
  • 8.1 Solutions à blanc
    4
  • 8.2 Étalons
    4
  • 8.3 Courbe d'étalonnage
    5
  • 8.4 Témoin de niveau d'activité phytasique
    6
  • 8.5 Prises d'essai d'aliments pour animaux
    6
  • 9 Calculs
    7
  • 9.1 Formule de la courbe d'étalonnage
    7
  • 9.2 Calcul de l'activité phytasique
    8
  • 9.3 Correction relative à la pureté et à la teneur en eau de l'acide phytique
    9
  • 9.4 Interférence avec les valeurs de blanc
    10
  • 10 Fidélité
    10
  • 10.1 Limite de détection et limite de quantification
    10
  • 10.2 Essai interlaboratoires
    10
  • 10.3 Répétabilité
    10
  • 10.4 Reproductibilité
    10
  • 11 Rapport d'essai
    11
  • Annexe A (informative) Résultats de l'étude interlaboratoires
    12
  • Bibliographie
    13
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