NF EN ISO 5983-2

October 2009

Standard Current

Animal feeding stuffs - Determination of nitrogen content and calculation of crude protein content - Part 2 : block digestion and steam distillation method

ISO 5983-2:2009 specifies a method for the determination of nitrogen content of animal feeding stuffs according to the Kjeldahl method, and a method for the calculation of the crude protein content. It is suitable for use as a semi-micro rapid routine method using block digestion, copper catalyst, and steam distillation into boric acid. The method is applicable to the determination of greater than 0,5 % mass fraction Kjeldahl nitrogen in animal feeding stuffs, pet foods, and their raw materials. The method does not measure oxidized forms of nitrogen nor heterocyclic nitrogen compounds. The method does not distinguish between protein nitrogen and non-protein nitrogen.

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Main informations

Collections

National standards and national normative documents

Publication date

October 2009

Number of pages

22 p.

Reference

NF EN ISO 5983-2

ICS Codes

65.120 Animal feeding stuffs

Classification index

V18-100-2

Print number

1 - 15/09/2009

International kinship

ISO 5983-2:2009

European kinship

EN ISO 5983-2:2009

Sumary

Animal feeding stuffs - Determination of nitrogen content and calculation of crude protein content - Part 2 : block digestion and steam distillation method

ISO 5983-2:2009 specifies a method for the determination of nitrogen content of animal feeding stuffs according to the Kjeldahl method, and a method for the calculation of the crude protein content.

It is suitable for use as a semi-micro rapid routine method using block digestion, copper catalyst, and steam distillation into boric acid.

The method is applicable to the determination of greater than 0,5 % mass fraction Kjeldahl nitrogen in animal feeding stuffs, pet foods, and their raw materials.

The method does not measure oxidized forms of nitrogen nor heterocyclic nitrogen compounds.

The method does not distinguish between protein nitrogen and non-protein nitrogen.

Replaced standards (1)

NF EN ISO 5983-2

December 2005

Standard Cancelled

Animal feeding stuffs - Determination of nitrogen content and calculation of crude protein content - Part 2 : block digestion/steam distillation method

Le présent document spécifie une méthode de routine semi-micro rapide détermination de la teneur en azote des aliments pour animaux selon la méthode de Kjeldahl et une méthode de calcul de la teneur en protéine brute. Cette méthode est utilisable pour des teneurs en azote supérieure à 0,5 %.

Table of contents

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Avant-propos
iv
1 Domaine d'application
1
2 Références normatives
1
3 Termes et définitions
2
4 Principe
2
5 Réactifs
2
6 Appareillage
4
7 Échantillonnage
5
8 Préparation de l'échantillon pour essai
5
9 Mode opératoire
5
9.1 Généralités
5
9.2 Prise d'essai
5
9.3 Détermination
5
9.4 Essai à blanc
7
9.5 Essais de récupération
7
10 Calcul et expression des résultats
8
10.1 Calcul
8
10.2 Calcul de la teneur en protéines brutes
8
10.3 Expression des résultats de teneur en protéines brutes
9
11 Fidélité
9
11.1 Essais interlaboratoires
9
11.2 Répétabilité
9
11.3 Reproductibilité
9
12 Rapport d'essai
10
Annexe A (informative) Résultats des essais interlaboratoires
11
Annexe B (informative) Résultats d'un essai d'aptitude; comparaison de la détermination par virage coloré et de la détermination potentiométrique du point d'arrêt d'un titrage
15
Bibliographie
16

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