NF EN ISO 6647-2

NF EN ISO 6647-2

August 2020
Standard Current

Rice - Determination of amylose content - Part 2 : spectrophotometric routine method without defatting procedure and with calibration from rice standards

This document specifies two simplified routine methods for the determination of the amylose mass fraction of milled rice, non-parboiled. The main difference between the two methods is the dispersion procedure: method A specifies hot dispersion, and method B specifies cold dispersion.Both methods are applicable to rice with an amylose mass fraction higher than 5 %.NOTE These methods describe simplified procedures for the preparation of samples, which are frequently used in routine laboratories. The methods use the same reagents as the reference method (see ISO 6647-1), but omit the defatting step. Rice samples where the amylose mass fraction has been determined by the reference method are used as standards.

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Main informations

Collections

National standards and national normative documents

Publication date

August 2020

Number of pages

18 p.

Reference

NF EN ISO 6647-2

ICS Codes

67.060   Cereals, pulses and derived products

Classification index

V03-734-2

Print number

1

International kinship

ISO 6647-2:2020

European kinship

EN ISO 6647-2:2020
Sumary
Rice - Determination of amylose content - Part 2 : spectrophotometric routine method without defatting procedure and with calibration from rice standards

This document specifies two simplified routine methods for the determination of the amylose mass fraction of milled rice, non-parboiled. The main difference between the two methods is the dispersion procedure: method A specifies hot dispersion, and method B specifies cold dispersion.

Both methods are applicable to rice with an amylose mass fraction higher than 5 %.

NOTE These methods describe simplified procedures for the preparation of samples, which are frequently used in routine laboratories. The methods use the same reagents as the reference method (see ISO 6647-1), but omit the defatting step. Rice samples where the amylose mass fraction has been determined by the reference method are used as standards.

Replaced standards (1)
NF EN ISO 6647-2
July 2015
Standard Cancelled
Rice - Determination of amylose content - Part 2 : routine methods

ISO 6647-2:2015 specifies a simplified routine method for the determination of the amylose content of milled, non-parboiled rice in the range from 1 % to 30 %. Rice samples for which the amylose content has been determined by the reference method size exclusion chromatography (SEC) are used as standards to generate the calibration curve.

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Réactifs
  • 6 Appareillage
  • 7 Échantillonnage
  • 8 Mode opératoire
  • 9 Expression des résultats
  • 10 Fidélité
  • 11 Rapport d'essai
  • Annexe A Résultats d'un essai interlaboratoires pour la méthode de routine
  • Bibliographie
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