NF EN ISO 6888-1
Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 1 : technique using Baird-Parker agar medium
La présente partie de l'ISO 6888 spécifie une méthode horizontale pour le dénombrement des staphylocoques à coagulase positive dans les produits destinés à la consommation humaine ou à l'alimentation animale, par comptage des colonies obtenues sur milieu solide (milieu de Baird-Parker) après incubation en aérobiose à 35°C ou 37°C.
La présente partie de l'ISO 6888 spécifie une méthode horizontale pour le dénombrement des staphylocoques à coagulase positive dans les produits destinés à la consommation humaine ou à l'alimentation animale, par comptage des colonies obtenues sur milieu solide (milieu de Baird-Parker) après incubation en aérobiose à 35°C ou 37°C.
<p>This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (Baird-Parker medium)<sup>[10]</sup> after aerobic incubation at 34 °C to 38 °C and coagulase confirmation.</p> <p>This document is applicable to:</p> <p>— products intended for human consumption;</p> <p>— products intended for animal feeding;</p> <p>— environmental samples in the area of food and feed production, handling, and</p> <p>— samples from the primary production stage.</p> <p>This horizontal method was originally developed for the examination of all samples belonging to the food chain.</p> <p>Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.</p> <p>Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on <em>Staphylococcus</em> spp (e.g. <em>S. xylosus</em>) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:</p> <p>— staphylococci forming atypical colonies on a Baird-Parker agar medium;</p> <p>— background flora that can obscure the colonies being sought.</p> <p>Nevertheless, both this document and ISO 6888-2 are given equivalent status.</p>
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1Domaine d'application 1
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2Références normatives 1
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3Termes et définitions 1
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4Principe 2
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5Diluant et milieux de culture 2
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6Appareillage et verrerie 5
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7Échantillonnage 6
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8Préparation de l'échantillon pour essai 6
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9Mode opératoire 6
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10Expression des résultats 8
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11Fidélité 10
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12Rapport d'essai 10
- Bibliographie11
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