NF EN ISO 7932

NF EN ISO 7932

July 2005
Standard Current

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 °C

ISO 7932:2004 specifies a horizontal method for the enumeration of viable Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. In order to have a practicable test method, the confirmatory stage has been restricted to the typical aspect on MYP agar and the haemolysis test. Thus the term presumptive has been introduced in order to acknowledge the fact that the confirmatory stage does not enable the distinction of B. cereus from other closely related but less commonly encountered Bacillus species, such as B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides. An additional motility test may help to differentiate B. cereus from B. anthracis in cases where the presence of the latter is suspected.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2005

Number of pages

23 p.

Reference

NF EN ISO 7932

ICS Codes

07.100.30   Food microbiology

Classification index

V08-023

Print number

1 - 07/07/2005

International kinship

ISO 7932:2004

European kinship

EN ISO 7932:2004
Sumary
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 °C

ISO 7932:2004 specifies a horizontal method for the enumeration of viable Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

In order to have a practicable test method, the confirmatory stage has been restricted to the typical aspect on MYP agar and the haemolysis test. Thus the term presumptive has been introduced in order to acknowledge the fact that the confirmatory stage does not enable the distinction of B. cereus from other closely related but less commonly encountered Bacillus species, such as B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides. An additional motility test may help to differentiate B. cereus from B. anthracis in cases where the presence of the latter is suspected.

Replaced standards (1)
NF EN ISO 7932
March 1998
Standard Cancelled
Microbiology. General guidance for the enumeration of Bacillus cereus. Colony-count technique at 30 degrees Celsius.

Table of contents
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  • Avant-propos
    iv
  • 0
    Introduction v
  • 1
    Domaine d'application 1
  • 2
    Références normatives 1
  • 3
    Termes et définitions 1
  • 4
    Principe 2
  • 5
    Diluant, milieux de culture et réactifs 2
  • 6
    Appareillage et verrerie 4
  • 7
    Échantillonnage 5
  • 8
    Préparation de l'échantillon pour l'essai 5
  • 9
    Mode opératoire 5
  • 9.1
    Prise d'essai, suspension mère et dilutions 5
  • 9.2
    Ensemencement et incubation 5
  • 9.3
    Comptage des colonies 6
  • 9.4 Confirmation
    6
  • 10
    Expression des résultats 7
  • 10.1
    Comptage des colonies de B. cereus présomptifs 7
  • 10.2
    Aucune colonie 7
  • 10.3 Fidélité
    7
  • 11
    Rapport d'essai 8
  • Annexe A (normative) Limites de confiance des estimations des petits nombres de colonies
    9
  • Annexe B (informative) Résultats de l'essai interlaboratoires
    10
  • Bibliographie
    13
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