NF EN ISO 8692

NF EN ISO 8692

May 2012
Standard Current

Water quality - Fresh water algal growth inhibition test with unicellular green algae

ISO 8692:2012 specifies a method for the determination of the growth inhibition of unicellular green algae by substances and mixtures contained in water or by waste water. This method is applicable for substances that are easily soluble in water.With modifications to this method, as specified in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, heavy metals and waste water can be tested.A rapid algal growth inhibition screening test for waste water is described in an annex.An alternative test procedure with algae from algal beads, with direct measurement of algal growth in spectrophotometric cells, is described in an annex.

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Main informations

Collections

National standards and national normative documents

Publication date

May 2012

Number of pages

29 p.

Reference

NF EN ISO 8692

ICS Codes

13.060.70   Examination of biological properties of water

Classification index

T90-304

Print number

1 - 19/04/2012

International kinship

ISO 8692:2012

European kinship

EN ISO 8692:2012
Sumary
Water quality - Fresh water algal growth inhibition test with unicellular green algae

ISO 8692:2012 specifies a method for the determination of the growth inhibition of unicellular green algae by substances and mixtures contained in water or by waste water. This method is applicable for substances that are easily soluble in water.

With modifications to this method, as specified in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, heavy metals and waste water can be tested.

A rapid algal growth inhibition screening test for waste water is described in an annex.

An alternative test procedure with algae from algal beads, with direct measurement of algal growth in spectrophotometric cells, is described in an annex.

Replaced standards (1)
NF EN ISO 8692
May 2005
Standard Cancelled
Water quality - Freshwater algal growth inhibition test with unicellular green algae

ISO 8692:2004 specifies a method for the determination of the growth inhibition of unicellular green algae by substances and mixtures contained in water or by wastewater. The method is applicable for substances that are easily soluble in water. With modifications to this method, as described in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, heavy metals and waste water can be tested. A rapid algal growth inhibition screening test for wastewater is also included.

Table of contents
  • Avant-propos
    iv
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    2
  • 5 Réactifs et milieux
    3
  • 6 Appareillage
    4
  • 7 Mode opératoire
    5
  • 7.1 Préparation du milieu de croissance
    5
  • 7.2 Préparation de la préculture et de l'inoculum
    5
  • 7.3 Choix des concentrations de la solution pour essai
    6
  • 7.4 Préparation de l'échantillon pour essai et des solutions mères
    6
  • 7.5 Préparation des lots d'essai et des témoins
    6
  • 7.6 Incubation
    7
  • 7.7 Mesurages
    7
  • 8 Critères de validité
    7
  • 9 Calculs
    8
  • 9.1 Représentation graphique des courbes de croissance
    8
  • 9.2 Calcul du pourcentage d'inhibition
    8
  • 9.3 Détermination de CE., (par exemple CEr10 et CEr50)
    9
  • 10 Expression des résultats
    9
  • 11 Interprétation des résultats
    9
  • 12 Fidélité
    9
  • 13 Rapport d'essai
    10
  • Annexe A (informative) Détection rapide de l'inhibition de la croissance algale par les eaux résiduaires
    12
  • Annexe B (informative) Mode opératoire avec des algues provenant de billes d'alginate, avec mesurage direct de la croissance algale dans des cellules spectrophotométriques
    15
  • Annexe C (informative) Mode opératoire d'immobilisation des algues dans des billes d'alginate
    21
  • Bibliographie
    23
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