NF ISO 11268-1

NF ISO 11268-1

December 2012
Standard Cancelled

Soil quality - Effects of pollutants on earthworms - Part 1 : determination of acute toxicity to Eisenia fetida/Eisenia andrei

1 Scope This part of ISO 11268 specifies one of the methods for evaluating the habitat function of soils and determining the acute toxicity of soil contaminants and chemicals to Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. It is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects on survival are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil). Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions. The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C. This method does not take into account the possible degradation of the substances or contaminants during the test.

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Main informations

Collections

National standards and national normative documents

Publication date

December 2012

Number of pages

25 p.

Reference

NF ISO 11268-1

ICS Codes

13.080.30   Biological properties of soils

Classification index

X31-252-1

Print number

1 - 14/12/2012

International kinship

Sumary
Soil quality - Effects of pollutants on earthworms - Part 1 : determination of acute toxicity to Eisenia fetida/Eisenia andrei

1 Scope

This part of ISO 11268 specifies one of the methods for evaluating the habitat function of soils and determining the acute toxicity of soil contaminants and chemicals to Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. It is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials.

Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects on survival are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil).

Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions.

The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.

This method does not take into account the possible degradation of the substances or contaminants during the test.

Standard replaced by (1)
NF EN ISO 11268-1
October 2015
Standard Current
Soil quality - Effects of pollutants on earthworms - Part 1 : determination of acute toxicity to Eisenia fetida/Eisenia andrei

<p>1 Scope</p> <p>This part of ISO 11268 specifies one of the methods for evaluating the habitat function of soils and determining the acute toxicity of soil contaminants and chemicals to Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. It is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials.</p> <p>Effects of substances are assessed using a standard soil, preferably a defined artificial soil substrate. For contaminated soils, the effects on survival are determined in the test soil and in a control soil. According to the objective of the study, the control and dilution substrate (dilution series of contaminated soil) should be either an uncontaminated soil comparable to the soil sample to be tested (reference soil) or a standard soil (e.g. artificial soil).</p> <p>Information is provided on how to use this method for testing chemicals under temperate as well as under tropical conditions.</p> <p>The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.</p> <p>This method does not take into account the possible degradation of the substances or contaminants during the test.</p>

Table of contents
View the extract
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    3
  • 5 Réactifs et matériaux
    4
  • 6 Appareillage
    5
  • 7 Mode opératoire
    6
  • 7.1 Plan d'expérimentation
    6
  • 7.2 Préparation des mélanges d'essai
    7
  • 7.3 Introduction des vers de terre
    8
  • 7.4 Conditions d'essai et mesurages
    8
  • 7.5 Substance de référence
    9
  • 8 Calcul et expression des résultats
    9
  • 8.1 Calcul
    9
  • 8.2 Expression des résultats
    9
  • 9 Validité de l'essai
    9
  • 10 Analyse statistique
    10
  • 10.1 Généralités
    10
  • 10.2 Essais à une seule concentration
    10
  • 10.3 Essais à plusieurs concentrations
    11
  • 11 Rapport d'essai
    12
  • Annexe A (informative) Détermination de la toxicité aiguë des substances chimiques (en particulier les pesticides) sur les vers de terre dans des conditions d'essai tropicales
    13
  • Annexe B (informative) Élevage d'Eisenia fetida et d'Eisenia andrei
    15
  • Annexe C (informative) Détermination de la capacité de rétention d'eau
    16
  • Annexe D (informative) Informations de base sur l'effet aigu de l'acide borique sur les vers de terre
    17
  • Bibliographie
    18
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