NF ISO 13495

NF ISO 13495

February 2014
Standard Current

Foodstuffs - Principles of selection and criteria of validation for the varietal identification methods using specific nucleic acid

ISO 13495:2013 specifies molecular tools for generating molecular profiles of varieties of plant species, enabling varietal identification, i.e. confirmation of identity in relation to one or more references. ISO 13495:2013 is applicable to various matrices, seeds, leaves, roots, industrial products composed of only one variety. Matrices presented in the form of mixtures of varieties (such as purees, compotes, flours) are excluded from the scope of ISO 13495:2013. It does not deal with genetic purity.

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Main informations

Collections

National standards and national normative documents

Publication date

February 2014

Number of pages

19 p.

Reference

NF ISO 13495

ICS Codes

67.050   General methods of tests and analysis for food products

Classification index

V03-045

Print number

1

International kinship

ISO 13495:2013
Sumary
Foodstuffs - Principles of selection and criteria of validation for the varietal identification methods using specific nucleic acid

ISO 13495:2013 specifies molecular tools for generating molecular profiles of varieties of plant species, enabling varietal identification, i.e. confirmation of identity in relation to one or more references.

ISO 13495:2013 is applicable to various matrices, seeds, leaves, roots, industrial products composed of only one variety. Matrices presented in the form of mixtures of varieties (such as purees, compotes, flours) are excluded from the scope of ISO 13495:2013. It does not deal with genetic purity.

Replaced standards (1)
NF V03-045
March 2009
Standard Cancelled
Selection principles and validation criteria for varietal identification methods using specific nucleic acid analyses

Le présent document décrit des outils moléculaires, permettant d'établir des profils moléculaires de variétés d'espèces végétales dans un but d'identification variétale, c'est-à-dire la confirmation d'identité par rapport à une ou des référence(s). Ce document ne traite pas de la pureté génétique.

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 3.1 Termes relatifs à la variété
    1
  • 3.2 Termes relatifs à l'extraction et à la purification de l'ADN/ARN
    2
  • 3.3 Termes relatifs à l'amplification des acides nucléiques par PCR
    2
  • 3.4 Termes relatifs à la détection
    3
  • 3.5 Termes relatifs aux témoins
    3
  • 3.6 Termes relatifs aux marqueurs
    4
  • 4 Assurance qualité des résultats d'essai
    4
  • 5 Choix des méthodes
    6
  • 6 Choix des marqueurs
    6
  • 6.1 Critères généraux
    6
  • 6.2 Choix du jeu de marqueurs
    6
  • 6.3 Marqueurs SSR
    6
  • 6.4 Polymorphisme mononucléotidique (SNP)
    7
  • 7 Échantillons pour laboratoire
    7
  • 7.1 Échantillon
    7
  • 7.2 Taille de l'échantillon
    7
  • 7.3 Matériau de référence
    7
  • 8 Validation en laboratoire
    8
  • 8.1 Généralités
    8
  • 8.2 Critères de validation intralaboratoire
    8
  • 8.3 Critères de validation interlaboratoires
    9
  • 9 Interprétation et expression des résultats
    11
  • 9.1 Généralités
    11
  • 9.2 Système de notation des données
    11
  • 9.3 Analyse des résultats
    11
  • 9.4 Expression des résultats
    12
  • 10 Rapport d'essai
    12
  • Bibliographie
    13
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