NF ISO 16212
Cosmetics - Microbiology - Enumeration of yeast and mould
ISO 16212:2008 gives general guidelines for enumeration of yeast and mould present in cosmetics, by counting the colonies on selective agar medium after aerobic incubation.In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which ISO 16212:2008 is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.
ISO 16212:2008 gives general guidelines for enumeration of yeast and mould present in cosmetics, by counting the colonies on selective agar medium after aerobic incubation.
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which ISO 16212:2008 is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.
ISO 16212:2008 gives general guidelines for enumeration of yeast and mould present in cosmetics, by counting the colonies on selective agar medium after aerobic incubation. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which ISO 16212:2008 is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.
- Avant-proposiv
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1 Domaine d'application1
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2 Références normatives1
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3 Termes et définitions1
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4 Principes2
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4.1 Généralités2
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4.2 Dénombrement sur gélose en boites de Pétri2
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4.3 Filtration sur membrane2
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5 Diluants, agents neutralisants et milieux de culture3
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5.1 Généralités3
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5.2 Diluants neutralisants et diluants3
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5.3 Diluant pour la suspension de levure (solution tryptone chlorure de sodium)4
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5.4 Milieux de culture4
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6 Appareillage et verrerie5
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7 Souches de micro-organismes5
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8 Manipulation des produits cosmétiques et des échantillons de laboratoire6
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9 Mode opératoire6
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9.1 Recommandation générale6
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9.2 Préparation de la suspension mère6
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9.3 Méthodes de dénombrement6
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10 Dénombrement des colonies (méthode de dénombrement sur gélose et méthode par filtration sur membrane)8
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11 Expression des résultats8
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11.1 Méthode de calcul pour le dénombrement sur gélose des boites de Pétri8
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11.2 Interprétation9
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12 Neutralisation des propriétés fongicides du produit11
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12.1 Généralités11
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12.2 Préparation de l'inoculum11
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12.3 Validation des méthodes de dénombrement11
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13 Rapport d'essai12
- Annexe A (informative) Autres diluants neutralisants13
- Annexe B (informative) Autres diluants15
- Annexe C (informative) Autres milieux de culture16
- Annexe D (informative) Neutralisants de l'activité fongicide des conservateurs et des liquides de rinçage18
- Bibliographie19
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