NF ISO 18415
Cosmetics - Microbiology - Detection of specified and non-specified microorganisms
ISO 18415:2007 gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products.Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans.The method described in ISO 18415:2007 is based on the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required.In ISO 18415:2007 specific indications are given for identification of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Other microorganisms that grow under the conditions described in this International Standard, may be identified by using suitable tests according to a general scheme.
ISO 18415:2007 gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products.
Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans.
The method described in ISO 18415:2007 is based on the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required.
In ISO 18415:2007 specific indications are given for identification of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Other microorganisms that grow under the conditions described in this International Standard, may be identified by using suitable tests according to a general scheme.
ISO 18415:2007 gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. The method described in ISO 18415:2007 is based on the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required. In ISO 18415:2007 specific indications are given for identification of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Other microorganisms that grow under the conditions described in this International Standard, may be identified by using suitable tests according to a general scheme.
- Avant-proposiv
- Introductionv
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1 Domaine d'application1
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2 Références normatives1
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3 Termes et définitions2
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4 Principe3
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5 Diluants et milieux de culture3
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5.1 Généralités3
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5.2 Diluant pour la suspension microbienne (solution de tryptone et de chlorure de sodium)4
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5.3 Milieux de culture4
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6 Appareillage et verrerie6
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7 Souches de micro-organismes6
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8 Manipulation des produits cosmétiques et des échantillons de laboratoire7
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9 Mode opératoire7
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9.1 Recommandations générales7
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9.2 Préparation de la suspension initiale dans le milieu liquide d'enrichissement7
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9.3 Incubation de la suspension initiale8
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9.4 Isolement des micro-organismes spécifiés et non spécifiés8
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9.5 Mode opératoire d'identification du micro-organisme spécifié: Pseudomonas aeruginosa8
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9.6 Mode opératoire d'identification du micro-organisme spécifié: Escherichia coli8
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9.7 Mode opératoire d'identification du micro-organisme spécifié: Staphylococus aureus9
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9.8 Mode opératoire d'identification du micro-organisme spécifié: Candida albicans9
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9.9 Mode opératoire d'identification des micro-organismes non spécifiés10
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10 Expression des résultats10
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10.1 Détection des micro-organismes spécifiés10
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10.2 Détection de micro-organismes non spécifiés11
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10.3 Absence de micro-organismes11
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11 Neutralisation des propriétés antimicrobiennes du produit11
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11.1 Généralités11
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11.2 Préparation de l'inoculum11
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11.3 Validation de la méthode de détection par enrichissement11
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12 Rapport d'essai13
- Annexe A (informative) Schéma général pour l'identification des micro-organismes14
- Annexe B (informative) Autres milieux15
- Annexe C (informative) Neutralisants de l'activité antimicrobienne des conservateurs et des liquides de rinçage18
- Bibliographie19
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