NF ISO 18415

NF ISO 18415

November 2007
Standard Cancelled

Cosmetics - Microbiology - Detection of specified and non-specified microorganisms

ISO 18415:2007 gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products.Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans.The method described in ISO 18415:2007 is based on the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required.In ISO 18415:2007 specific indications are given for identification of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Other microorganisms that grow under the conditions described in this International Standard, may be identified by using suitable tests according to a general scheme.

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Main informations

Collections

National standards and national normative documents

Publication date

November 2007

Number of pages

27 p.

Reference

NF ISO 18415

ICS Codes

07.100.40   Cosmetics microbiology

Classification index

T75-606

Print number

2 - 01/01/2008

International kinship

Sumary
Cosmetics - Microbiology - Detection of specified and non-specified microorganisms

ISO 18415:2007 gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products.

Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans.

The method described in ISO 18415:2007 is based on the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required.

In ISO 18415:2007 specific indications are given for identification of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Other microorganisms that grow under the conditions described in this International Standard, may be identified by using suitable tests according to a general scheme.

Standard replaced by (1)
NF EN ISO 18415
August 2011
Standard Cancelled
Cosmetics - Microbiology - Detection of specified and non-specified microorganisms

ISO 18415:2007 gives general guidelines for the detection and identification of specified microorganisms in cosmetic products as well as for the detection and identification of other kinds of aerobic mesophilic non-specified microorganisms in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. Most of them considered as specified microorganisms include one or more of the following species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. The method described in ISO 18415:2007 is based on the detection of microbial growth in a non-selective liquid medium (enrichment broth) suitable to detect microbial contamination, followed by isolation of microorganisms on non-selective agar media. Other methods can be appropriate depending on the level of detection required. In ISO 18415:2007 specific indications are given for identification of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Candida albicans. Other microorganisms that grow under the conditions described in this International Standard, may be identified by using suitable tests according to a general scheme.

Table of contents
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    3
  • 5 Diluants et milieux de culture
    3
  • 5.1 Généralités
    3
  • 5.2 Diluant pour la suspension microbienne (solution de tryptone et de chlorure de sodium)
    4
  • 5.3 Milieux de culture
    4
  • 6 Appareillage et verrerie
    6
  • 7 Souches de micro-organismes
    6
  • 8 Manipulation des produits cosmétiques et des échantillons de laboratoire
    7
  • 9 Mode opératoire
    7
  • 9.1 Recommandations générales
    7
  • 9.2 Préparation de la suspension initiale dans le milieu liquide d'enrichissement
    7
  • 9.3 Incubation de la suspension initiale
    8
  • 9.4 Isolement des micro-organismes spécifiés et non spécifiés
    8
  • 9.5 Mode opératoire d'identification du micro-organisme spécifié: Pseudomonas aeruginosa
    8
  • 9.6 Mode opératoire d'identification du micro-organisme spécifié: Escherichia coli
    8
  • 9.7 Mode opératoire d'identification du micro-organisme spécifié: Staphylococus aureus
    9
  • 9.8 Mode opératoire d'identification du micro-organisme spécifié: Candida albicans
    9
  • 9.9 Mode opératoire d'identification des micro-organismes non spécifiés
    10
  • 10 Expression des résultats
    10
  • 10.1 Détection des micro-organismes spécifiés
    10
  • 10.2 Détection de micro-organismes non spécifiés
    11
  • 10.3 Absence de micro-organismes
    11
  • 11 Neutralisation des propriétés antimicrobiennes du produit
    11
  • 11.1 Généralités
    11
  • 11.2 Préparation de l'inoculum
    11
  • 11.3 Validation de la méthode de détection par enrichissement
    11
  • 12 Rapport d'essai
    13
  • Annexe A (informative) Schéma général pour l'identification des micro-organismes
    14
  • Annexe B (informative) Autres milieux
    15
  • Annexe C (informative) Neutralisants de l'activité antimicrobienne des conservateurs et des liquides de rinçage
    18
  • Bibliographie
    19
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