NF ISO 19344

NF ISO 19344

May 2016
Standard Current

Milk and milk products - Starter cultures, probiotics and fermented products - Quantification of lactic acid bacteria by flow cytometry

ISO 19344:2015 specifies a standardized method for the quantification of active and/or total lactic acid bacteria and probiotic strains in starter cultures used in dairy products by means of flow cytometry. The method is also applicable to probiotics used in dairy products and to fermented milk products such as yogurts containing primarily lactic acid bacteria.This International Standard does not apply to taxonomical differentiation of bacteria. Due to its non-specificity, the method may quantify other bacteria than those within the scope of this International Standard, when present in the sample. This may lead to overestimation of the counts.The minimum bacterial cell concentration in the sample before applying this standardized method depends on the dilution rates used in the individual protocols. Typically 106 cells per gram or ml are considered within the minimum range.

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Main informations

Collections

National standards and national normative documents

Publication date

May 2016

Number of pages

35 p.

Reference

NF ISO 19344

ICS Codes

67.100.10   Milk and processed milk products

Classification index

V04-397

Print number

1

International kinship

ISO 19344:2015
Sumary
Milk and milk products - Starter cultures, probiotics and fermented products - Quantification of lactic acid bacteria by flow cytometry

ISO 19344:2015 specifies a standardized method for the quantification of active and/or total lactic acid bacteria and probiotic strains in starter cultures used in dairy products by means of flow cytometry. The method is also applicable to probiotics used in dairy products and to fermented milk products such as yogurts containing primarily lactic acid bacteria.

This International Standard does not apply to taxonomical differentiation of bacteria. Due to its non-specificity, the method may quantify other bacteria than those within the scope of this International Standard, when present in the sample. This may lead to overestimation of the counts.

The minimum bacterial cell concentration in the sample before applying this standardized method depends on the dilution rates used in the individual protocols. Typically 106 cells per gram or ml are considered within the minimum range.

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Diluants et réactifs
  • 6 Appareillage
  • 7 Échantillonnage
  • 8 Préparation de l'échantillon pour essai
  • 9 Mode opératoire
  • 10 Calcul et expression des résultats
  • 11 Facteurs critiques affectant les résultats
  • 12 Fidélité
  • 13 Rapport d'essai
  • Annexe A Schéma des protocoles de coloration
  • Annexe B Exemple de calcul de la dilution appropriée de l'échantillon
  • Annexe C Essai interlaboratoires
  • Bibliographie
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