NF ISO 20813

NF ISO 20813

October 2019
Standard Cancelled

Molecular biomarker analysis - Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) - General requirements and definitions

This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods.The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods.It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.

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Main informations

Collections

National standards and national normative documents

Publication date

October 2019

Number of pages

31 p.

Reference

NF ISO 20813

ICS Codes

67.050   General methods of tests and analysis for food products

Classification index

V03-510

Print number

1

International kinship

ISO 20813:2019
Sumary
Molecular biomarker analysis - Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) - General requirements and definitions

This document specifies minimum requirements of performance characteristics for the detection of nucleic acid sequences (DNA) by molecular methods, such as the polymerase chain reaction (PCR), including different post-PCR detection methods, real-time PCR, single and/or multiple probe-based detection techniques as well as the combination of such methods.

The document is applicable to the detection, identification and quantification of DNA from animal species of higher and lower taxonomic groups in foodstuffs, and the validation of applicable methods.

It is applicable to mammals, birds, reptiles, amphibians, fishes, molluscs, crustaceans and insects. Typical examples for each are listed in Annex A.

Standard replaced by (1)
NF EN ISO 20813
December 2022
Standard Current
Molecular biomarker analysis - Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) - General requirements and definitions

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Caractéristiques de performances des méthodes
  • 5 Validation par un seul laboratoire
  • 6 Étude interlaboratoires
  • 7 Exigences générales du laboratoire et du mode opératoire
  • 8 Rapport d'essai
  • Annexe A Liste des espèces typiques utilisées pour les essais d'inclusivité et d'exclusivité
  • Annexe B Exemples de méthodes de conversion des unités des nombres de copies d'ADN en rapport de masses
  • Bibliographie
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