NF ISO 20963

NF ISO 20963

September 2005
Standard Cancelled

Soil quality - Effects of pollutants on insect larvae (Oxythyrea funesta) - Determination of acute toxicity

ISO 20963:2005 describes a method for the determination of the effects of contaminated soils and substances on the survival of the larvae of Oxythyrea funesta. The larvae are exposed to the pollutants by cuticular and alimentary uptake.For contaminated soils, the effects on the survival are determined in the test soil and in a control soil. Depending on the objectives of the study, the control and dilution substrates (dilution series of contaminated soil) are either uncontaminated soil comparable to the soil sample to be tested or artificial soil substrate. Effects of substances are assessed using a defined artificial soil substrate.ISO 20963:2005 is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,001 33 Pa at 25 °C.This method does not take into account the possible degradation of the substances or pollutants during the test.

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Main informations

Collections

National standards and national normative documents

Publication date

September 2005

Number of pages

19 p.

Reference

NF ISO 20963

ICS Codes

13.080.30   Biological properties of soils

Classification index

X31-204

Print number

1 - 07/09/2005

International kinship

ISO 20963:2005
Sumary
Soil quality - Effects of pollutants on insect larvae (Oxythyrea funesta) - Determination of acute toxicity

ISO 20963:2005 describes a method for the determination of the effects of contaminated soils and substances on the survival of the larvae of Oxythyrea funesta. The larvae are exposed to the pollutants by cuticular and alimentary uptake.

For contaminated soils, the effects on the survival are determined in the test soil and in a control soil. Depending on the objectives of the study, the control and dilution substrates (dilution series of contaminated soil) are either uncontaminated soil comparable to the soil sample to be tested or artificial soil substrate. Effects of substances are assessed using a defined artificial soil substrate.

ISO 20963:2005 is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,001 33 Pa at 25 °C.

This method does not take into account the possible degradation of the substances or pollutants during the test.

Standard replaced by (1)
NF EN ISO 20963
October 2011
Standard Current
Soil quality - Effects of pollutants on insect larvae (Oxythyrea funesta) - Determination of acute toxicity

ISO 20963:2005 describes a method for the determination of the effects of contaminated soils and substances on the survival of the larvae of Oxythyrea funesta. The larvae are exposed to the pollutants by cuticular and alimentary uptake. For contaminated soils, the effects on the survival are determined in the test soil and in a control soil. Depending on the objectives of the study, the control and dilution substrates (dilution series of contaminated soil) are either uncontaminated soil comparable to the soil sample to be tested or artificial soil substrate. Effects of substances are assessed using a defined artificial soil substrate. ISO 20963:2005 is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,001 33 Pa at 25 °C. This method does not take into account the possible degradation of the substances or pollutants during the test.

Table of contents
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    2
  • 5 Environnement de l'essai
    3
  • 6 Réactifs
    3
  • 7 Appareillage
    4
  • 8 Mode opératoire
    4
  • 8.1 Protocole d'essai
    4
  • 8.2 Préparation des mélanges d'essai
    5
  • 8.3 Préparation du récipient témoin
    6
  • 8.4 Apport de nourriture
    6
  • 8.5 Introduction du matériel biologique
    6
  • 8.6 Conditions d'essai et mesurages
    6
  • 8.7 Substance de référence
    7
  • 9 Expression des résultats
    7
  • 9.1 Calculs
    7
  • 9.2 Expression des résultats
    8
  • 10 Validité de l'essai
    8
  • 11 Rapport d'essai
    8
  • Annexe A (informative) Exemple de technique d'élevage pour Oxythyrea funesta
    10
  • Annexe B (informative) Résultats de l'essai circulaire réalisé en France
    12
  • Bibliographie
    13
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