NF ISO 21149

NF ISO 21149

June 2006
Standard Cancelled

Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria

ISO 21149:2006 gives general guidelines for enumeration and detection of mesophilic aerobic bacteria present in cosmetics:by counting the colonies on agar medium after aerobic incubation, orby checking the absence of bacterial growth after enrichment.Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise validated. If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.

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National standards and national normative documents

Publication date

June 2006

Number of pages

30 p.

Reference

NF ISO 21149

ICS Codes

07.100.40   Cosmetics microbiology

Classification index

T75-602

Print number

1 - 26/05/2006

International kinship

ISO 21149:2006
Sumary
Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria

ISO 21149:2006 gives general guidelines for enumeration and detection of mesophilic aerobic bacteria present in cosmetics:

  • by counting the colonies on agar medium after aerobic incubation, or
  • by checking the absence of bacterial growth after enrichment.

Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise validated.

If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.

Standard replaced by (1)
NF EN ISO 21149
September 2009
Standard Cancelled
Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria

ISO 21149:2006 gives general guidelines for enumeration and detection of mesophilic aerobic bacteria present in cosmetics: by counting the colonies on agar medium after aerobic incubation, orby checking the absence of bacterial growth after enrichment. Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise validated. If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.

Table of contents
  • Avant-propos
    iv
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principes
    2
  • 4.1 Généralités
    2
  • 4.2 Dénombrement sur gélose en boites de Pétri
    2
  • 4.3 Filtration sur membrane
    2
  • 4.4 Détection des bactéries par enrichissement
    3
  • 5 Diluants, agents neutralisants et milieux de culture
    3
  • 5.1 Généralités
    3
  • 5.2 Diluants neutralisants et diluants
    3
  • 5.3 Diluant pour la suspension bactérienne (solution tryptone chlorure de sodium)
    4
  • 5.4 Milieux de culture
    4
  • 6 Matériel et verrerie
    7
  • 7 Souches de micro-organismes
    7
  • 8 Manipulation des produits cosmétiques et des échantillons de laboratoire
    7
  • 9 Mode opératoire
    8
  • 9.1 Recommandations générales
    8
  • 9.2 Préparation de la suspension mère
    8
  • 9.3 Méthodes de dénombrement
    8
  • 9.4 Enrichissement
    9
  • 10 Dénombrement des colonies (méthodes de dénombrement sur gélose et par filtration sur membrane)
    10
  • 11 Détection de croissance (méthode par enrichissement)
    10
  • 12 Expression des résultats
    10
  • 12.1 Méthode de calcul pour le dénombrement sur gélose en boites de Pétri
    10
  • 12.2 Interprétation
    11
  • 12.3 Exemples
    12
  • 12.4 Détection après enrichissement
    14
  • 13 Neutralisation des propriétés antimicrobiennes du produit
    14
  • 13.1 Généralités
    14
  • 13.2 Préparation de l'inoculum
    14
  • 13.3 Validation des méthodes de dénombrement
    14
  • 13.4 Validation de la méthode de détection par enrichissement
    16
  • 13.5 Interprétation des résultats de validation
    16
  • 14 Rapport d'essai
    17
  • Annexe A (informative) Autres diluants neutralisants
    18
  • Annexe B (informative) Autres diluants
    20
  • Annexe C (informative) Autres milieux de culture
    21
  • Annexe D (informative) Neutralisants de l'activité antimicrobienne des conservateurs et des liquides de rinçage
    25
  • Bibliographie
    26
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