NF ISO 21160

NF ISO 21160

February 2019
Standard Current

Cigarettes - Determination of selected carbonyls in the mainstream smoke of cigarettes - Method using high performance liquid chromatography

This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones in mainstream smoke using reversed phase HPLC-UV/DAD. This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette using reversed phase HPLC-UV/DAD.

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Main informations

Collections

National standards and national normative documents

Publication date

February 2019

Number of pages

23 p.

Reference

NF ISO 21160

ICS Codes

65.160   Tobacco, tobacco products and related equipment

Classification index

V37-201

Print number

1

International kinship

ISO 21160:2018
Sumary
Cigarettes - Determination of selected carbonyls in the mainstream smoke of cigarettes - Method using high performance liquid chromatography

This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones in mainstream smoke using reversed phase HPLC-UV/DAD.

This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette using reversed phase HPLC-UV/DAD.

Table of contents
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  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Appareillage
  • 6 Réactifs
  • 7 Préparation
  • 8 Échantillonnage
  • 9 Préparation des produits du tabac
  • 10 Production d'échantillons - Fumage de cigarettes
  • 11 Analyse de l'échantillon
  • 12 Répétabilité et reproductibilité
  • 13 Rapport d'essai
  • Annexe A Recristallisation de la 2,4-dinitrophénylhydrazine
  • Annexe B Exemple de préparation de solutions d'étalonnage
  • Annexe C Chromatogramme CLHP type d'une solution d'étalonnage d'un mélange de carbonyles
  • Annexe D Chromatogramme CLHP d'un extrait de carbonyles de DNPH du courant principal de fumée de cigarette
  • Bibliographie
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