NF ISO 22030

NF ISO 22030

June 2005
Standard Cancelled

Soil quality - Biological methods - Chronic toxicity in higher plants

ISO 22030:2005 describes a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions. Two species are recommended: a rapid-cycling variant of turnip rape (Brassica rapa CrGC syn. Rbr) and oat (Avena sativa). The duration of test should be sufficient to include chronic endpoints that demonstrate the reproductive capability of the test plants. By using natural test soils, e.g. from contaminated sites or remediated soils, and by comparing the development of the test plants in these soils with reference or standard control soils, the test is applicable to assess soil quality, especially the function of the soil as a habitat for plants. This method can be modified to allow use of the chronic plant assay for the testing of chemicals incorporated into soil. By preparing a dilution series of a test substance in standard control soils, the same endpoints can be measured to assess the chronic toxicity of chemicals. This method is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.

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Main informations

Collections

National standards and national normative documents

Publication date

June 2005

Number of pages

27 p.

Reference

NF ISO 22030

ICS Codes

13.080.30   Biological properties of soils

Classification index

X31-206

Print number

1 - 25/05/2005

International kinship

Sumary
Soil quality - Biological methods - Chronic toxicity in higher plants

ISO 22030:2005 describes a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions. Two species are recommended: a rapid-cycling variant of turnip rape (Brassica rapa CrGC syn. Rbr) and oat (Avena sativa). The duration of test should be sufficient to include chronic endpoints that demonstrate the reproductive capability of the test plants.

By using natural test soils, e.g. from contaminated sites or remediated soils, and by comparing the development of the test plants in these soils with reference or standard control soils, the test is applicable to assess soil quality, especially the function of the soil as a habitat for plants.

This method can be modified to allow use of the chronic plant assay for the testing of chemicals incorporated into soil. By preparing a dilution series of a test substance in standard control soils, the same endpoints can be measured to assess the chronic toxicity of chemicals.

This method is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.

Standard replaced by (1)
NF EN ISO 22030
October 2011
Standard Current
Soil quality - Biological methods - Chronic toxicity in higher plants

<p>ISO 22030:2005 describes a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions. Two species are recommended: a rapid-cycling variant of turnip rape (<i>Brassica rapa</i> CrGC syn. Rbr) and oat (<i>Avena sativa</i>). The duration of test should be sufficient to include chronic endpoints that demonstrate the reproductive capability of the test plants.</p> <p>By using natural test soils, e.g. from contaminated sites or remediated soils, and by comparing the development of the test plants in these soils with reference or standard control soils, the test is applicable to assess soil quality, especially the function of the soil as a habitat for plants.</p> <p>This method can be modified to allow use of the chronic plant assay for the testing of chemicals incorporated into soil. By preparing a dilution series of a test substance in standard control soils, the same endpoints can be measured to assess the chronic toxicity of chemicals. </p> <p>This method is not applicable to volatile substances, i.e. substances for which Henry's constant or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 0,013 3 Pa at 25 °C.</p>

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Prinriaux
    4
  • 5.1 Plancipe
    4
  • 5 Matétes testées
    4
  • 5.2 Sol et stockage du sol
    5
  • 5.3 Substance de référence
    7
  • 6 Équipement
    7
  • 7 Méthodes
    9
  • 7.1 Montage expérimental
    9
  • 7.2 Essai préalable
    9
  • 7.3 Essai préliminaire (détermination de la plage de concentrations)
    9
  • 7.4 Essai final
    9
  • 7.5 Préparation des pots
    9
  • 7.6 Préparation des graines
    10
  • 7.7 Conditions de croissance
    10
  • 7.8 Début de l'essai
    10
  • 7.9 Manipulation au cours de l'essai
    11
  • 7.10 Mesure des points de mesure
    12
  • 7.11 Récapitulatif et calendrier de l'essai
    13
  • 8 Critères de validité
    14
  • 9 Évaluation des résultats
    14
  • 9.1 Présentation des mesures
    14
  • 9.2 Analyse statistique
    15
  • 10 Rapport d'essai
    16
  • Annexe A (informative) Essais des produits chimiques dans le sol
    17
  • Bibliographie
    18
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