Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Escherichia coli - Most probable number technique

ISO 7251:2005 gives general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 °C, then at 44 °C. This International Standard is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2005

Number of pages

21 p.

Reference

NF ISO 7251

ICS Codes

07.100.30 Food microbiology

Classification index

V08-020

Print number

1 - 01/08/2005

International kinship

ISO 7251:2005

Sumary

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Escherichia coli - Most probable number technique

ISO 7251:2005 gives general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 °C, then at 44 °C. This International Standard is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

Replaced standards (2)

NF ISO 7251

September 1994

Standard Cancelled

Microbiology. General guidance for enumeration of presumptive {Escherichia} {coli}. Most probable number technique.

NF ISO 11866-1

September 1997

Standard Cancelled

Milk and milk products. Enumeration of presumptive Escherichia coli. Part 1 : most probable number technique.

Table of contents

Avant-propos
iv
Introduction
v
1 Domaine d'application
1
2 Références normatives
1
3 Termes et définitions
2
4 Principe
2
4.1 Méthode de détection
2
4.2 Méthode de dénombrement
2
5 Diluant, milieux de culture et réactif
3
6 Appareillage et verrerie
5
7 Échantillonnage
5
8 Préparation de l'échantillon pour essai
6
9 Procédure
6
9.1 Méthode de détection
6
9.2 Méthode de dénombrement
7
10 Expression des résultats
8
10.1 Méthode de détection
8
10.2 Méthode de dénombrement
8
11 Rapport d'essai
8
Annexe A (normative) Table NPP
10
Bibliographie
13

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