NF U47-016
Animal health analysis methods - Detection of antibodies against equine influenza by the complement fixation test
Le présent document s'inscrit dans un ensemble de normes de méthodes d'analyses en santé animale. Il décrit une technique de mise en évidence d'anticorps dirigés contre le virus de la grippe dans le sérum des équidés. La technique utilisée est la fixation du complément.
1 Domaine d'application 2 Références 3 Termes et définitions 4 Principe et réaction 5 Diluants, milieux de culture, réactifs et autres produits 6 Appareillage et verrerie 7 Échantillonnage 8 Préparation de l'échantillon pour analyse 9 Mode opératoire 10 Conditions de conservation et d'élimination des échantillons Annexe A (normative) Titrage du sérum hémolytique Annexe B (normative) Constitution d'un stock de complément Annexe C (normative) Préparation de la gamme d'hémolyse Annexe D (normative) Titrage du complément Annexe E (normative) Titrage de l'antigène Bibliographie
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