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National standards and national normative documents

Publication date

June 2010

Number of pages

23 p.

Reference

NF U47-025

ICS Codes

11.220 Veterinary medicine

65.020.30 Animal husbandry and breeding

Classification index

U47-025

Print number

1 - 07/06/2010

Sumary

Animal health analysis methods - Detection of antibodies against classical swine fever by virus neutralisation test and immunochemistry on cell cultures (IF or IP)

Le présent document s'inscrit dans un ensemble de normes de méthodes d'analyses en santé animale. Il décrit une technique de recherche dans le sérum de suidés d'anticorps neutralisant le virus de la Peste Porcine Classique. La technique utilisée est celle de la neutralisation virale.

Replaced standards (1)

NF U47-025

October 2001

Standard Cancelled

Animal health analysis methods - Detection of antibodies against classical swine fever by virus neutralisation test and immunochemistry on cell cultures (IF or IP)

Le présent document s'inscrit dans un ensemble de normes de méthodes d'analyses en santé animale. Il décrit une technique de recherche dans le sérum de suidés d'anticorps neutralisant le virus de la Peste Porcine Classique. La technique est celle de la neutralisation virale.

Standard replaced by (1)

NF U47-025

October 2001

Standard Cancelled

Animal health analysis methods - Detection of antibodies against classical swine fever by virus neutralisation test and immunochemistry on cell cultures (IF or IP)

Le présent document s'inscrit dans un ensemble de normes de méthodes d'analyses en santé animale. Il décrit une technique de recherche dans le sérum de suidés d'anticorps neutralisant le virus de la Peste Porcine Classique. La technique est celle de la neutralisation virale.

Table of contents

Introduction
6
1 Domaine d'application
7
2 Références normatives
7
2.1 Références normatives et réglementaires
7
2.2 Références réglementaires
7
3 Termes et définitions
7
4 Principe et réaction
9
5 Diluants, milieux de culture, réactifs et autres produits
9
5.1 Diluants
9
5.2 Milieux de culture et additifs
11
5.3 Cellules
12
5.4 Virus
12
5.5 Sérums de contrôle
13
5.6 Autres produits
14
6 Appareillage et verrerie
15
7 Échantillonnage
15
8 Préparation de l'échantillon pour analyse
15
9 Mode opératoire
15
9.1 Épreuve
15
9.2 Validation et lecture
19
9.3 Interprétation
20
10 Conditions de conservation et d'élimination des échantillons
21
11 Restitution des résultats
21
12 Fidélité
21
13 Rapport d'analyse
21
Annexe A (normative) Méthode de calcul d'un titre viral ou d'un titre en anticorps
22
Bibliographie
23

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