Animal health analysis methods - Detection of antibodies against equine arteritis virus by virus neutralisation test on cell culture

Introduction 5

1 Domaine d'application 5

2 Références normatives 5

3 Termes et définitions 5

4 Principe et réaction 6

5 Diluants, milieux de culture, réactifs et autres produits 6

5.1 Diluants 6

5.1.1 Eau 6

5.2 Milieux de culture et additifs 6

5.2.1 Milieu de base 6

5.2.2 Sérum 7

5.2.3 Antibiotiques 7

5.2.4 Milieu de culture complet 7

5.3 Cellules 7

5.4 Virus 7

5.4.1 Souches virales 7

5.4.2 Préparation de la suspension stock de virus 8

5.4.3 Titrage de la suspension stock de virus 8

5.5 Sérums de contrôle 9

5.5.1 Sérum de contrôle positif 9

5.5.2 Sérum de contrôle négatif 9

5.6 Produits 9

6 Appareillage et verrerie 9

7 Échantillonnage 9

8 Préparation de l'échantillon pour analyse 9

9 Mode opératoire 10

9.1 Épreuve 10

9.1.1 Contrôles 10

9.1.2 Témoins 10

9.2 Validation et lecture 11

9.2.1 Lecture 11

9.2.2 Validation 11

9.2.3 Lecture des échantillons testés 11

9.3 Interprétation 12

9.3.1 Expression des résultats 12

9.3.2 Interprétation des résultats 12

10 Conditions de conservation et d'élimination des échantillons 12

11 Restitution des résultats 12

12 Fidélité 13

13 Rapport d'analyse 13

Annexe A (normative) Méthode de calcul d'un titre viral ou d'un titre en anticorps 14

Bibliographie 15

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