Foodstuffs - Determination of vitamin C by high performance liquid chromatography

1 Domaine d'application 5

2 Références normatives 5

3 Principe 5

4 Réactifs 5

4.1 Généralités 5

4.2 Produits chimiques et solutions 5

4.3 Substances étalons 6

4.4 Solutions mères 6

4.5 Solutions d'étalonnage 7

5 Appareillage 7

5.1 Généralités 7

5.4 Colonne pour CLHP 7

5.5 Dispositif de filtrage 8

6 Mode opératoire 8

6.1 Généralités 8

6.2 Préparation de l'échantillon pour essai 8

6.3 Préparation de la solution d'échantillon pour essai 8

6.4 Identification 9

6.5 Dosage 9

7 Calcul 9

8 Fidélité 10

8.1 Généralités 10

8.2 Répétabilité 10

8.3 Reproductibilité 10

9 Rapport d'essai 11

Annexe A (informative) Exemple de chromatogramme 12

Annexe B (informative) Données relatives à la fidélité 13

Bibliographie 14

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