XP CEN/TS 15790
XP CEN/TS 15790

XP CEN/TS 15790

April 2010
Standard Current

Animal feeding stuffs - PCR typing of probiotic strains of Saccharomyces cerevisiae (yeast)

Le présent document définit une méthode d'amplification en chaîne par polymérase (ACP) permettant l'identification des souches de levure probiotiques de Saccharomyces cerevisiae.Il présente également une méthode d'extraction d'un ADN de bonne qualité à partir de la levure.Il rentre dans le cadre de la Directive européenne 70/373/CE.

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Main informations

Collections

National standards and national normative documents

Publication date

April 2010

Number of pages

13 p.

Reference

XP CEN/TS 15790

ICS Codes

07.100.30   Food microbiology
65.120   Animal feeding stuffs

Classification index

V18-240

Print number

1 - mai 2010

European kinship

CEN/TS 15790:2008
Sumary
Animal feeding stuffs - PCR typing of probiotic strains of Saccharomyces cerevisiae (yeast)

Le présent document définit une méthode d'amplification en chaîne par polymérase (ACP) permettant l'identification des souches de levure probiotiques de Saccharomyces cerevisiae. Il présente également une méthode d'extraction d'un ADN de bonne qualité à partir de la levure. Il rentre dans le cadre de la Directive européenne 70/373/CE.
Table of contents
  • Avant-propos
    3
  • Introduction
    4
  • 1 Domaine d'application
    4
  • 2 Principe
    4
  • 3 Réactifs
    4
  • 3.1 ACP
    4
  • 3.2 Électrophorèse sur gel
    5
  • 4 Appareillage
    6
  • 4.1 ACP
    6
  • 4.2 Électrophorèse sur gel
    6
  • 5 Mode opératoire
    6
  • 5.1 Réaction d'amplification en chaîne par polymérase
    6
  • 5.2 Thermocycleur
    7
  • 5.3 Électrophorèse sur gel d'agarose
    7
  • 6 Analyse des résultats
    8
  • Annexe A (informative) Exemple de gel - ACP des souches probiotiques de Saccharomyces cerevisiae
    9
  • Annexe B (informative) Données de validation de l'essai interlaboratoires européen
    10
  • Bibliographie
    11
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