XP T90-412

XP T90-412

June 2006
Standard Cancelled

Water quality - Detection and enumeration of pathogenic staphylococci - Method by membrane filtration

Le présent document décrit une méthode de recherche et de dénombrement des staphylocoques pathogènes dans les eaux. Il est applicable aux eaux filtrables (eaux de piscines, eaux de consommation humaine, eaux des établissements de soins, etc.).

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Main informations

Collections

National standards and national normative documents

Publication date

June 2006

Number of pages

14 p.

Reference

XP T90-412

ICS Codes

07.100.20   Microbiology of water

Classification index

T90-412

Print number

1 - 21/06/2006
Sumary
Water quality - Detection and enumeration of pathogenic staphylococci - Method by membrane filtration

Le présent document décrit une méthode de recherche et de dénombrement des staphylocoques pathogènes dans les eaux. Il est applicable aux eaux filtrables (eaux de piscines, eaux de consommation humaine, eaux des établissements de soins, etc.).
Standard replaced by (1)
NF T90-412
June 2016
Standard Current
Water quality - Enumeration of pathogenic staphylococci - Method by membrane filtration

Le présent document décrit une méthode de dénombrement des staphylocoques pathogènes à coagulase positive dans les eaux. Il est applicable aux eaux filtrables (eaux de piscines, eaux de consommation humaine, eaux des établissements de soins, etc.).

Table of contents
  • Introduction
    5
  • 1 Domaine d'application
    5
  • 2 Références normatives
    5
  • 3 Termes et définitions
    5
  • 4 Principe
    6
  • 5 Échantillonnage
    6
  • 6 Appareillage et verrerie
    6
  • 7 Milieux de culture et réactifs
    6
  • 7.1 Généralités
    6
  • 7.2 Milieu de Chapman au mannitol
    7
  • 7.3 Milieu gélosé de Baird Parker (BP)
    7
  • 7.4 Milieu gélosé au plasma de lapin et au fibrinogène (BP + RPF)
    9
  • 7.5 Bouillon coeur-cervelle (BCC)
    10
  • 7.6 Plasma de lapin
    10
  • 7.7 Réactif pour test d'agglutination au latex
    10
  • 8 Mode opératoire
    10
  • 8.1 Prise d'essai
    10
  • 8.2 Ensemencement et incubation
    10
  • 8.3 Confirmation des colonies
    10
  • 9 Expression des résultats
    12
  • 10 Rapport d'essai
    12
  • Annexe A
    (informative) Exemple de résultat avec repiquage 13
  • Bibliographie
    14
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