XP T90-722-2

Water quality - Molecular, physiological and behavioral measurements in the mammal (amphipod crustacean) - Part 2 : measurement of reproduction markers

Le présent document décrit une méthode pour mesurer des marqueurs individuels liés à la reproduction chez des gammares femelles calibrés selon la taille et à des stades de mue spécifiques : nombre total d'embryons et nombre d'embryons anormaux ainsi que le nombre total d'ovocytes et la taille ovocytaire moyenne. Cette méthode est applicable aux espèces de Gammaridae, marines et d'eaux douces pour des gammares directement prélevés in natura ; pour des gammares exposés au laboratoire à une substance chimique, un mélange de substances chimiques, une eau naturelle, un effluent, ou encore un sédiment naturel ou enrichi ; ou pour des gammares encagés in situ en milieux aquatiques. La méthode est applicable sur le terrain pour les eaux continentales de surface : rivières, fleuves, canaux et plans d'eau.

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