Microbioly of food and animal feeding stuffs - Enumeration of microorganisms by colony-count technique obtained at 30 °C spirale method

1 Domaine d'application 5

2 Références normatives 5

3 Termes et définitions 5

4 Principe 6

5 Diluant en milieux de culture 6

5.1 Généralités 6

5.2 Diluant 6

5.3 Gélose pour dénombrement (PCA) 6

5.4 Gélose non nutritive, dite "Gélose blanche" 7

6 Appareillage et verrerie 8

7 Échantillonnage 8

8 Préparation de l'échantillon pour essai 8

9 Mode opératoire 9

9.1 Prise d'essai suspension mère et dilutions 9

9.2 Ensemencement et incubation 9

9.3 Comptage des colonies 9

10 Expression des résultats 9

10.1 Cas général 9

10.2 Cas des prélèvements de surface 10

11 Rapport d'essai 10

Bibliographie 11

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