NF EN ISO 7937

NF EN ISO 7937

February 2005
Standard Cancelled

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony-count technique

ISO 7937:2004 describes a horizontal method for the enumeration of viable Clostridium perfringens. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

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National standards and national normative documents

Publication date

February 2005

Number of pages

27 p.

Reference

NF EN ISO 7937

ICS Codes

07.100.30   Food microbiology

Classification index

V08-019

Print number

1 - 08/02/2005

International kinship

European kinship

EN ISO 7937:2004
Sumary
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony-count technique

ISO 7937:2004 describes a horizontal method for the enumeration of viable Clostridium perfringens. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

Replaced standards (1)
NF EN 13401
January 2003
Standard Cancelled
Microbiologie of food and animal feeding stuffs - Horizontal method for enumeration of Clostridium perfringens - Colony-count technique

Le présent document décrit une méthode horizontale pour le dénombrement de Clostridium perfringens revivifiables dans les produits destinés à la consommation humaine ou à l'alimentation animale.

Standard replaced by (1)
NF EN ISO 15213-2
November 2023
Standard Current
Microbiology of the food chain - Horizontal method for the detection and enumeration of Clostridium spp. - Part 2 : enumeration of Clostridium perfringens by colony-count technique

<p class="MsoBodyText"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">This document specifies the enumeration of <em style="mso-bidi-font-style: normal;">Clostridium (C.) perfringens</em> by colony-count technique.</span></p> <p class="MsoBodyText"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">This document is applicable to:</span></p> <p class="ListContinue1"><span lang="EN-GB">—<span style="mso-tab-count: 1;">    </span>products intended for human consumption;</span></p> <p class="ListContinue1"><span lang="EN-GB">—<span style="mso-tab-count: 1;">    </span>products for feeding animals;</span></p> <p class="ListContinue1"><span lang="EN-GB">—<span style="mso-tab-count: 1;">    </span>environmental samples in the area of food and feed production and handling;</span></p> <p class="ListContinue1"><span lang="EN-GB">—<span style="mso-tab-count: 1;">    </span>samples from the primary production stage.</span></p> <p class="Note"><span lang="EN-GB">NOTE<span style="mso-tab-count: 1;">            </span>This method has been validated in an interlaboratory study for the following food categories:</span></p> <p class="ListContinue1-"><span lang="EN-GB">—<span style="mso-tab-count: 1;">     </span>ready-to-eat, ready-to-reheat meat products;</span></p> <p class="ListContinue1-"><span lang="EN-GB">—<span style="mso-tab-count: 1;">     </span>eggs and egg products (derivates);</span></p> <p class="ListContinue1-"><span lang="EN-GB">—<span style="mso-tab-count: 1;">     </span>processed fruits and vegetables;</span></p> <p class="ListContinue1-"><span lang="EN-GB">—<span style="mso-tab-count: 1;">     </span>infant formula and infant cereals;</span></p> <p class="ListContinue1-"><span lang="EN-GB">—<span style="mso-tab-count: 1;">     </span>multi-component foods or meal components.</span></p> <p class="Notecontinued"><span lang="EN-GB">It has also been validated for the following other categories:</span></p> <p class="ListContinue1-"><span lang="EN-GB">—<span style="mso-tab-count: 1;">     </span>pet food and animal feed;</span></p> <p class="ListContinue1-"><span lang="EN-GB">—<span style="mso-tab-count: 1;">     </span>environmental samples (food or feed production).</span></p> <p class="Notecontinued"><span lang="EN-GB">As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see </span><span class="citesec"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt; color: black; mso-color-alt: windowtext;">Clause 11</span></span><span lang="EN-GB"> and </span><span class="citeapp"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt; color: black; mso-color-alt: windowtext;">Annex C</span></span><span lang="EN-GB">. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category.</span></p> <p class="MsoBodyText"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.</span></p> <p class="MsoBodyText"><span lang="EN-GB" style="mso-bidi-font-size: 12.0pt;">This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.</span></p>

Table of contents
View the extract
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    2
  • 5 Diluant, milieux de culture et réactifs
    2
  • 6 Appareillage et verrerie
    7
  • 7 Échantillonnage
    8
  • 8 Préparation de l'échantillon pour essai
    8
  • 9 Mode opératoire
    8
  • 10 Expression des résultats
    10
  • 11 Rapport d'essai
    12
  • Annexe A (informative) Résultats d'essai interlaboratoires
    13
  • Bibliographie
    17
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