NF EN ISO 12966-2

NF EN ISO 12966-2

June 2017
Standard Current

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2 : preparation of methyl esters of fatty acids

ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids. It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF3 transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides. Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

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Main informations

Collections

National standards and national normative documents

Publication date

June 2017

Number of pages

25 p.

Reference

NF EN ISO 12966-2

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-233-2

Print number

1

International kinship

ISO 12966-2:2017

European kinship

EN ISO 12966-2:2017
Sumary
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2 : preparation of methyl esters of fatty acids

ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids.

It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely:

a) a "rapid" transmethylation procedure under alkaline conditions;

b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions;

c) a BF3 transmethylation procedure;

d) an alternative procedure using acid-catalysed transmethylation of glycerides.

Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).

Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Replaced standards (1)
NF EN ISO 12966-2
June 2011
Standard Cancelled
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2 : preparation of methyl esters of fatty acids

Le présent document spécifie quatre méthodes de préparation des esters méthyliques d'acides gras à partir des corps gras d'origines animale et végétale, des acides gras et des savons. Afin de répondre aux différentes exigences, les quatre méthodes de méthylation décrites sont les suivantes : la méthode de transméthylation "rapide" en conditions alcalines ; la méthode de transméthylation/méthylation "générale" en conditions alcalines et acides séquentielles ; la méthode de transméthylation au BF3 ; la méthode alternative reposant sur la transméthylation par catalyse acide de glycérides.

Table of contents
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  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Réactions
  • 5 Méthodologie
  • Annexe A (informative) Méthode de chromatographie sur couche mince pour vérifier l'état complet de la dérivatisation
  • Bibliographie
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