NF EN ISO 12966-2

NF EN ISO 12966-2

June 2011
Standard Cancelled

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2 : preparation of methyl esters of fatty acids

ISO 12966-2:2011 specifies methods of preparing the methyl esters of fatty acids.It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF3 transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides.Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GC), thin-layer chromatography (TLC), and infrared spectrometry (IR).

See more
View the extract
Main informations

Collections

National standards and national normative documents

Publication date

June 2011

Number of pages

26 p.

Reference

NF EN ISO 12966-2

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-233-2

Print number

1 - 16/06/2011

International kinship

ISO 12966-2:2011

European kinship

EN ISO 12966-2:2011
Sumary
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2 : preparation of methyl esters of fatty acids

ISO 12966-2:2011 specifies methods of preparing the methyl esters of fatty acids.

It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF3 transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides.

Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GC), thin-layer chromatography (TLC), and infrared spectrometry (IR).

Replaced standards (3)
NF EN ISO 5509
June 2000
Standard Cancelled
Animal and vegetable fats and oils - Preparation of methyl esters of fatty acids

Le présent document spécifie trois méthodes de préparation des esters méthyliques d'acides gras à partir des corps gras d'origines animale et végétale, des acides gras et des savons.Afin de répondre aux différentes prescriptions, les trois méthodes de méthylation décrites sont les suivantes :- la méthode au trifluorure de bore (BF3)- la méthode à l'hydroxyde de triméthylsulfonium (TMSH)- la méthode de transestérification.

NF EN ISO 5508
June 1995
Standard Cancelled
Animal and vegetable fats and oils. Analysis by gas chromatography of methyl esters of fatty acids.

NF EN ISO 15304
May 2002
Standard Cancelled
Animal and vegetable fats and oils - Determination of the content of trans fatty acid isomers of vegetable fats and oils - Gas chromatographic method

Le présent document spécifie une méthode de détermination de la teneur en isomères trans d'acides gras de corps gras d'origine végétale par chromatographie en phase gazeuse utilisant des colonnes capillaires. Cette méthode est particulièrement conçue pour évaluer la teneur en isomères trans obtenus lors du raffinage ou de l'hydrogénation de ces corps gras.

Standard replaced by (1)
NF EN ISO 12966-2
June 2017
Standard Current
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 2 : preparation of methyl esters of fatty acids

ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids. It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely: a) a "rapid" transmethylation procedure under alkaline conditions; b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions; c) a BF3 transmethylation procedure; d) an alternative procedure using acid-catalysed transmethylation of glycerides. Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

Table of contents
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Réactions
    1
  • 4 Méthodologie
    2
  • 4.1 Préparation de l'échantillon pour essai
    2
  • 4.2 Méthode rapide
    2
  • 4.3 Méthode générale
    4
  • 4.4 Transméthylation à l'aide d'un catalyseur au trifluorure de bore (BF3)
    6
  • 4.5 Transméthylation par catalyse acide des glycérides
    10
  • Annexe A (informative) Méthode de chromatographie sur couche mince pour vérifier l'état complet de la dérivatisation
    12
  • Bibliographie
    16
ZOOM ON ... the Requirements department
To comply with a standard, you need to quickly understand its issues in order to determine its impact on your activity.

The Requirements department helps you quickly locate within the normative text:
- mandatory clauses to satisfy,
- non-essential but useful clauses to know, such as permissions and recommendations.

The identification of these types of clauses is based on the document “ISO / IEC Directives, Part 2 - Principles and rules of structure and drafting of ISO documents ”as well as on a constantly enriched list of verbal forms.

With Requirements, quickly access the main part of the normative text!

With Requirements, quickly access the main part of the normative text!
Need to identify, monitor and decipher standards?

COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.

Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!

Request your free, no-obligation live demo

I discover COBAZ