NF EN ISO 15216-1

NF EN ISO 15216-1

May 2017
Standard Current

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1 : method for quantification

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

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Main informations

Collections

National standards and national normative documents

Publication date

May 2017

Number of pages

56 p.

Reference

NF EN ISO 15216-1

ICS Codes

07.100.30   Food microbiology

Classification index

V08-670-1

Print number

1

International kinship

ISO 15216-1:2017

European kinship

EN ISO 15216-1:2017
Sumary
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1 : method for quantification

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

Replaced standards (1)
XP CEN ISO/TS 15216-1
June 2013
Standard Cancelled
Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 1 : method for quantification

ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

Table of contents
  • Avant-propos
  • 1
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Réactifs
  • 6 Matériel et consommables
  • 7 Échantillonnage
  • 8 Mode opératoire
  • 9 Interprétation des résultats
  • 10 Expression des résultats
  • 11 Fidélité
  • 12 Rapport d'essai
  • Annexe A (normative) Diagramme de mode opératoire
  • Annexe B (normative) Composition et préparation des réactifs et des tampons
  • Annexe C (informative) Mélanges réactionnels (MasterMix) pour la RT-PCR en temps réel et paramètres de cycles
  • Annexe D (informative) Amorces et sondes d'hydrolyse de la RT-PCR en temps réel pour la détection du VHA, du norovirus GI et GII et du mengovirus (témoin d'extraction)
  • Annexe E (informative) Multiplication de la souche MC0 du mengovirus utilisée comme témoin d'extraction
  • Annexe F (informative) Extraction d'ARN avec le système NucliSENS
  • Annexe G (informative) Préparation des contrôles ADNdb
  • Annexe H (informative) Préparation des ARN CE
  • Annexe I (informative) Disposition de plaque optique type
  • Annexe J (informative) Études de validation des méthodes et caractéristiques de performance
  • Bibliographie
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