XP CEN ISO/TS 15216-1

XP CEN ISO/TS 15216-1

June 2013
Standard Cancelled

Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 1 : method for quantification

ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

See more
View the extract
Main informations

Collections

National standards and national normative documents

Publication date

June 2013

Number of pages

43 p.

Reference

XP CEN ISO/TS 15216-1

ICS Codes

07.100.30   Food microbiology

Classification index

V08-670-1

Print number

1 - 11/07/2013

International kinship

ISO/TS 15216-1:2013

European kinship

CEN ISO/TS 15216-1:2013
Sumary
Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 1 : method for quantification

ISO/TS 15216-1:2013 describes a method for quantification of levels of HAV and NoV genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

Standard replaced by (1)
NF EN ISO 15216-1
May 2017
Standard Current
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1 : method for quantification

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

Table of contents
  • Avant-propos
    v
  • Introduction
    viii
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principe
    3
  • 4.1 Extraction de virus
    3
  • 4.2 Extraction d'ARN
    4
  • 4.3 Transcription inverse suivie d'une réaction de polymérisation en chaîne en temps réel (RT-PCR en temps réel)
    4
  • 4.4 Témoins
    4
  • 4.5 Résultats des essais
    5
  • 5 Réactifs
    5
  • 5.1 Généralités
    5
  • 5.2 Réactifs utilisés dans l'état
    5
  • 5.3 Réactifs préparés
    6
  • 6 Appareillage et matériaux
    7
  • 7 Échantillonnage
    9
  • 8 Mode opératoire
    9
  • 8.1 Exigences générales de laboratoire
    9
  • 8.2 Extraction de virus
    9
  • 8.3 Extraction d'ARN
    11
  • 8.4 RT PCR en temps réel
    12
  • 9 Interprétation des résultats
    14
  • 9.1 Généralités
    14
  • 9.2 Élaboration des courbes étalon
    14
  • 9.3 Calcul de l'efficacité de l'amplification
    14
  • 9.4 Calcul du rendement d'extraction
    15
  • 9.5 Quantification d'échantillon
    15
  • 9.6 Limite de détection théorique
    15
  • 10 Expression des résultats
    16
  • 11 Rapport d'essai
    16
  • Annexe A (normative) Diagramme de mode opératoire
    17
  • Annexe B (informative) Mélanges maîtres (MasterMix) de la technique RT PCR en temps réel et paramètres de cycles
    18
  • Annexe C (informative) Amorces et sondes d'hydrolyse de la technique RTPCR en temps réel pour la détection du VHA, du norovirus GI et GII et du virus Mengo(témoin de processus)
    19
  • Annexe D (informative) Croissance de la souche du virus Mengo MCo utilisé comme témoin de processus
    21
  • Annexe E (informative) Extraction d'ADN par l'utilisation du système NucliSens de chez BioMerieux
    22
  • Annexe F (normative) Composition et préparation des réactifs et des tampons
    24
  • Annexe G (informative) Préparations mères des témoins d'ADN double brin (ADNdb)
    26
  • Annexe H (informative) Préparations mères d'ADN témoin externe
    28
  • Annexe I (informative) Disposition de plaque optique type
    30
  • Bibliographie
    31
ZOOM ON ... the Requirements department
To comply with a standard, you need to quickly understand its issues in order to determine its impact on your activity.

The Requirements department helps you quickly locate within the normative text:
- mandatory clauses to satisfy,
- non-essential but useful clauses to know, such as permissions and recommendations.

The identification of these types of clauses is based on the document “ISO / IEC Directives, Part 2 - Principles and rules of structure and drafting of ISO documents ”as well as on a constantly enriched list of verbal forms.

With Requirements, quickly access the main part of the normative text!

With Requirements, quickly access the main part of the normative text!
Need to identify, monitor and decipher standards?

COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.

Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!

Request your free, no-obligation live demo

I discover COBAZ