NF EN ISO 15753
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons
ISO 15753:2006 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: a general method, and a method specific for coconut oil and short-chain vegetable oils.These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.
ISO 15753:2006 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: a general method, and a method specific for coconut oil and short-chain vegetable oils.
These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.
ISO 15753:2016 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: - a general method; - a specific method for coconut oil and vegetable oils with short-chain fatty acids. These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method. The quantification limit is 0,2 µg/kg for almost all compounds analysed, except for fluoranthene and benzo(g,h,i)perylene, where the quantification limit is 0,3 µg/kg, and indeno(1,2,3-c,d)pyrene, where the quantification limit is 1,0 µg/kg. NOTE The results for olive pomace oil in Annex B show that this method is not applicable to this type of oil. The precision data determined are very poor. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard.
- Avant-proposiv
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1Domaine d'application 1
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2Références normatives 1
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3Termes et définitions 1
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4Principe 2
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5Réactifs et matériaux 2
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6Appareillage 3
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7Échantillonnage 5
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8Préparation de l'échantillon 5
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9Mode opératoire pour la détermination des HAP à partir des corps gras: Méthode générale 5
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9.1Remarques préliminaires 5
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9.2Essai à blanc 5
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9.3Détermination des valeurs des taux de récupération (sans matrice) 6
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9.4Extraction (extraction liquide/liquide) 6
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9.5Purification sur des cartouches de phase greffée en C18 (extraction solide/liquide) 6
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9.6Purification sur des cartouches de phase greffée de Florisil (extraction solide/liquide) 7
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10Mode opératoire pour la détermination des HAP à partir des corps gras: Méthode spécifique pour l'huile de coprah 7
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10.1Première extraction (extraction liquide/liquide) 7
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10.2Deuxième extraction (extraction liquide/liquide) 8
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10.3Purification sur des cartouches de phase greffée en C18 (extraction solide/liquide) 8
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10.4Purification sur des cartouches de phase greffée de Florisil (extraction solide/liquide) 9
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11Chromatographie liquide à haute performance (CLHP) 9
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11.1Conditions de fonctionnement 9
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11.2Paramètres de détection 10
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11.3Analyse des échantillons et des étalons 12
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11.4Confirmation de la présence des HAP 13
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12Expression des résultats 13
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13Fidélité 13
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13.1Essai interlaboratoires 13
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13.2Répétabilité 14
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13.3Reproductibilité 14
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14Rapport d'essai 14
- Annexe A (informative) Valeurs des taux de récupération, schémas fonctionnels, chromatogrammes et séquences d'injection15
- Annexe B (informative) Résultats de l'essai interlaboratoires20
- Bibliographie23
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