NF EN ISO 20418-3

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3 : peptide detection using LC-MS without protein reduction

This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.

NOTE 1 The composition of non-animal hair fibres can be measured by ISO 1833 (all parts). Both results are combined to determine the total fibre composition.

The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827^[4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present.

NOTE 2 In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts)].

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