NF EN ISO 20418-3

NF EN ISO 20418-3

July 2020
Standard Current

Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3 : peptide detection using LC-MS without protein reduction

This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.NOTE 1 The composition of non-animal hair fibres can be measured by ISO 1833 (all parts). Both results are combined to determine the total fibre composition.The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827[4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present. NOTE 2 In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts)].

See more
View the extract
Main informations

Collections

National standards and national normative documents

Publication date

July 2020

Number of pages

38 p.

Reference

NF EN ISO 20418-3

ICS Codes

59.060.01   Textile fibres in general

Classification index

G06-007-3

Print number

1

International kinship

ISO 20418-3:2020

European kinship

EN ISO 20418-3:2020
Sumary
Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 3 : peptide detection using LC-MS without protein reduction

This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends (made of wool, cashmere, yak, alpaca, camel or angora) by LC-MS without protein reduction.

NOTE 1 The composition of non-animal hair fibres can be measured by ISO 1833 (all parts). Both results are combined to determine the total fibre composition.

The method is based on a preliminary identification, by light microscopy, of all fibres in the blend on the basis of their morphology, according to ISO/TR 11827[4]. It is not applicable if fibres of the same animal species (such as blends of cashmere and mohair) are present.

NOTE 2 In this case, the quantitative analysis is performed using microscopical analysis [for example, ISO 17751 (all parts)].

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Symboles et abréviations
  • 5 Principe
  • 6 Réactifs
  • 7 Appareillage
  • 8 Méthode d'essai
  • 9 Rapport d'essai
  • Annexe A Marqueurs peptidiques de fibres de cachemire, de laine de mouton et de yack
  • Annexe B Exemple de conditions d'analyse LC-MS
  • Annexe C Analyse de fibres de chameau, d'alpaga et d'angora
  • Annexe D Exemple de chromatogrammes de masse de marqueurs peptidiques
  • Annexe E Résultats de l'étude interlaboratoires
  • Bibliographie
ZOOM ON ... the Requirements department
To comply with a standard, you need to quickly understand its issues in order to determine its impact on your activity.

The Requirements department helps you quickly locate within the normative text:
- mandatory clauses to satisfy,
- non-essential but useful clauses to know, such as permissions and recommendations.

The identification of these types of clauses is based on the document “ISO / IEC Directives, Part 2 - Principles and rules of structure and drafting of ISO documents ”as well as on a constantly enriched list of verbal forms.

With Requirements, quickly access the main part of the normative text!

With Requirements, quickly access the main part of the normative text!
New: UPSELL service
- The UPSELL service allows you to easily update one of your standards.
- With a single click, add a new language, the Requirements or Redline+ service and add one or more additional users.
- Whether you are in the process of acquiring a standard or it is already available in your personal space, the UPSELL service is available at every stage to help you understand it and implement it within your organization.
Need to identify, monitor and decipher standards?

COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.

Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!

Request your free, no-obligation live demo

I discover COBAZ