NF EN ISO 21528-2

NF EN ISO 21528-2

July 2017
Standard Current

Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2 : Colony-count technique

ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to- products intended for human consumption and the feeding of animals, and- environmental samples in the area of primary production, food production and food handling.This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.The most probable number (MPN) technique, as included in ISO 21528‑1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2017

Number of pages

25 p.

Reference

NF EN ISO 21528-2

ICS Codes

07.100.30   Food microbiology

Classification index

V08-039-2

Print number

2 - 01/10/2021

International kinship

ISO 21528-2:2017

European kinship

EN ISO 21528-2:2017
Sumary
Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2 : Colony-count technique

ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to

- products intended for human consumption and the feeding of animals, and

- environmental samples in the area of primary production, food production and food handling.

This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.

The most probable number (MPN) technique, as included in ISO 21528‑1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.

Replaced standards (1)
NF ISO 21528-2
December 2004
Standard Cancelled
Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2 : colony-count method

ISO 21528-2:2004 specifies a method, without pre-enrichment, for the enumeration of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. Enumeration is carried out by counting colonies in a solid medium after incubation at 37 °C. This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.

Table of contents
  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Diluants, milieux de culture et réactifs
  • 6 Matériel et consommables
  • 7 Échantillonnage
  • 8 Préparation de l'échantillon pour essai
  • 9 Mode opératoire
  • 10 Expression des résultats
  • 11 Caractéristiques de performance de la méthode
  • 12 Rapport d'essai
  • 13 Assurance qualité
  • Annexe A (normative) Milieux de culture et réactifs
  • Annexe B (informative) Études de validation de la méthode et caractéristiques de performance
  • Bibliographie
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