NF ISO 21528-2

NF ISO 21528-2

December 2004
Standard Cancelled

Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2 : colony-count method

ISO 21528-2:2004 specifies a method, without pre-enrichment, for the enumeration of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.Enumeration is carried out by counting colonies in a solid medium after incubation at 37 °C.This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.

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Main informations

Collections

National standards and national normative documents

Publication date

December 2004

Number of pages

18 p.

Reference

NF ISO 21528-2

ICS Codes

07.100.30   Food microbiology

Classification index

V08-039-2

Print number

1 - 21/12/2004

International kinship

ISO 21528-2:2004
Sumary
Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2 : colony-count method

ISO 21528-2:2004 specifies a method, without pre-enrichment, for the enumeration of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

Enumeration is carried out by counting colonies in a solid medium after incubation at 37 °C.

This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.

Replaced standards (2)
NF ISO 7402
December 1993
Standard Cancelled
Microbiology. General guidance for the enumeration of {Enterobacteriaceae} without resuscitation. Mpn technique and colony count technique.

NF ISO 8523
February 1992
Standard Cancelled
Microbiology. General guidance for the detection of {Enterobacteriaceae} with pre-enrichment.

Standard replaced by (1)
NF EN ISO 21528-2
July 2017
Standard Current
Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2 : Colony-count technique

ISO 21528-2:2017 specifies a method for the enumeration of Enterobacteriaceae. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of primary production, food production and food handling. This technique is intended to be used when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample. The most probable number (MPN) technique, as included in ISO 21528‑1, is generally used when the number sought is expected to be below 100 per millilitre or per gram of test sample.

Table of contents
  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    2
  • 4.1 Préparation de la suspension initiale et des dilutions décimales
    2
  • 4.2 Isolement
    2
  • 4.3 Confirmation
    2
  • 4.4 Calcul
    2
  • 5 Diluants, milieux de culture et réactifs
    3
  • 6 Appareillage et verrerie
    5
  • 7 Échantillonnage
    5
  • 8 Préparation de l'échantillon soumis à l'essai
    6
  • 9 Mode opératoire
    6
  • 9.1 Généralités
    6
  • 9.2 Prise d'essai, suspension-mère et dilutions
    6
  • 9.3 Ensemencement et incubation
    6
  • 9.4 Comptage et sélection des colonies pour confirmation
    7
  • 9.5 Repiquage des colonies sélectionnées
    7
  • 9.6 Essais de confirmation biochimique
    7
  • 10 Expression des résultats
    7
  • 11 Rapport d'essai
    8
  • Annexe A (normative) Limites de confiance des estimations des petits nombres de colonies
    9
  • Bibliographie
    10
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