NF EN ISO 21572

May 2013

Standard Cancelled

Foodstuffs - Molecular biomarker analysis - Protein-based methods

ISO 21572:2013 provides general guidelines and performance criteria for methods for the detection and/or quantification of specific proteins or protein(s) of interest [POI(s)] in a specified matrix.These general guidelines address existing antibody based methods. Methods other than those described in Annex A or Annex B can also detect the POI. The same criteria as outlined in ISO 21572:2013 apply generally.

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Main informations

Collections

National standards and national normative documents

Publication date

May 2013

Number of pages

39 p.

Reference

NF EN ISO 21572

ICS Codes

07.080 Biology. Botany. Zoology

67.060 Cereals, pulses and derived products

Classification index

V03-021

Print number

International kinship

ISO 21572:2013

European kinship

EN ISO 21572:2013

Sumary

Foodstuffs - Molecular biomarker analysis - Protein-based methods

ISO 21572:2013 provides general guidelines and performance criteria for methods for the detection and/or quantification of specific proteins or protein(s) of interest [POI(s)] in a specified matrix.

These general guidelines address existing antibody based methods. Methods other than those described in Annex A or Annex B can also detect the POI. The same criteria as outlined in ISO 21572:2013 apply generally.

Replaced standards (1)

NF EN ISO 21572

July 2004

Standard Cancelled

Foodstuffs - Methods for the detection of genetically modified organisms and derived products - Protein based methods

Le présent document énonce des lignes directrices et des critères de performance généraux pour les méthodes de détection et/ou de quantification de protéines spécifiques dérivées de matériel végétal génétiquement modifié dans une matrice donnée. Ces directives générales concernent les méthodes axées sur des anticorps existants.

Standard replaced by (1)

NF EN ISO 21572

October 2019

Standard Current

Foodstuffs - Molecular biomarker analysis - Immunochemical methods for the detection and quantification of proteins

This document specifies performance criteria for immunochemical methods for the detection and/or quantification of a specific protein or protein(s) of interest [POI(s)] in a specified matrix. The methods discussed are applicable to the analysis of proteins from a variety of sample types. Some uses for these methods include, but are not limited to, analysing proteins involved in crop and food production, food processing, food marketing, food safety, biotechnology or disease indexing.

Table of contents

Avant-propos
iv
1 Domaine d'application
1
2 Références normatives
1
3 Termes et définitions
1
3.1 Termes généraux
1
3.2 Termes relatifs aux anticorps
2
3.3 Termes relatifs aux techniques
3
3.4 Termes relatifs au contrôle
3
3.5 Termes relatifs à la validation
4
4 Principe
6
5 Réactifs
6
6 Matériel de laboratoire
6
7 Échantillonnage
7
8 Mode opératoire
7
8.1 Généralités
7
8.2 Préparation de la solution d'échantillon
7
8.3 Extraction
7
8.4 Préparation des courbes d'étalonnage, des contrôles positifs et des matériaux de référence
7
8.5 Mode opératoire d'analyse
8
9 Interprétation et expression des résultats
8
9.1 Généralités
8
9.2 Analyses quantitative et semi-quantitative
8
9.3 Analyse qualitative
9
8 Paramètres spécifiques pouvant influer sur les résultats
9
10.1 Généralités
9
10.2 Considérations particulières
9
10.3 Applicabilité d'analyse
10
11 Méthode de confirmation
10
12 Rapport d'essai
11
Annexe A (informative) Détection d'une protéine par ELISA
12
Annexe B (informative) Détection de protéine(s) à l'aide de dispositifs d'immunochromatographie
23
Bibliographie
31

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