NF EN ISO 9936

NF EN ISO 9936

June 2016
Standard Current

Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol content by high-performance liquid chromatography

ISO 9936:2016 specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC). For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification. Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this International Standard. NOTE A suitable method involving a cold saponification procedure is described in Annex B for information only.

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National standards and national normative documents

Publication date

June 2016

Number of pages

22 p.

Reference

NF EN ISO 9936

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-239

Print number

1

International kinship

ISO 9936:2016

European kinship

EN ISO 9936:2016
Sumary
Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol content by high-performance liquid chromatography

ISO 9936:2016 specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC).

For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.

Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this International Standard.

NOTE A suitable method involving a cold saponification procedure is described in Annex B for information only.

Replaced standards (2)
NF EN ISO 9936
October 2006
Standard Cancelled
Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol content by high-performance liquid chromatography

Le présent document spécifie une méthode pour la détermination des teneurs en alpha-, bêta-, gamma- et delta-tocophérols et tocotriénols libres (appelés globalement tocols) des graisses et des huiles (appelés corps gras dans la suite du texte) d'origines animale et végétale, par chromatographie en phase liquide haute performance (CLHP). Pour les produits contenant des esters de tocophérols ou de tocotriénols, il est nécessaire qu'ils subissent une saponification préalable.

NF EN ISO 9936/A1
September 2011
Standard Cancelled
Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography - Amendment 1 : updating of reagents and confirmation of statistical data validity

Le présent document spécifie une méthode pour la détermination des teneurs en alpha-, bêta-, gamma- et delta-tocophérols et tocotriénols libres (appelés globalement tocols) des graisses et des huiles (appelés corps gras dans la suite du texte) d'origines animale et végétale, par chromatographie en phase liquide haute performance (CLHP). Cet amendement permet de mettre à jour certains réactifs proposés dans la norme NF EN ISO 9936:2006 et de confirmer la validité des données statistiques.

Table of contents
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  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Réactifs
  • 6 Appareillage
  • 7 Échantillonnage
  • 8 Préparation de l'échantillon pour essai
  • 9 Mode opératoire
  • 10 Expression des résultats
  • 11 Fidélité
  • 12 Rapport d'essai
  • Annexe A Exemples de chromatogrammes
  • Annexe B Saponification
  • Annexe C Résultats des essais interlaboratoires
  • Bibliographie
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