NF EN ISO 9936

October 2006

Standard Cancelled

Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol content by high-performance liquid chromatography

ISO 9936:2006 specifies a method for the determination of the contents of free alpha-, beta-, gamma- and delta-tocopherols and tocotrienols in animal and vegetable fats and oils by high-performance liquid chromatography. For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.

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Main informations

Collections

National standards and national normative documents

Publication date

October 2006

Number of pages

26 p.

Reference

NF EN ISO 9936

ICS Codes

67.200.10 Animal and vegetable fats and oils

Classification index

T60-239

Print number

2 - 01/06/2009

International kinship

ISO 9936:2006

European kinship

EN ISO 9936:2006

Sumary

Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol content by high-performance liquid chromatography

For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification.

Replaced standards (1)

NF ISO 9936

January 2002

Standard Cancelled

Animal and vegetable fats and oils - Determination of tocopherols and tocotrienolss contents - Method using high-performance liquid chromatography

Standard replaced by (1)

NF EN ISO 9936

June 2016

Standard Current

Animal and vegetable fats and oils - Determination of tocopherol and tocotrienol content by high-performance liquid chromatography

ISO 9936:2016 specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC). For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification. Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this International Standard. NOTE A suitable method involving a cold saponification procedure is described in Annex B for information only.

Table of contents

Avant-propos
iv
1 Domaine d'application
1
2 Références normatives
1
3 Termes et définitions
1
4 Principe
1
5 Réactifs
2
6 Appareillage
2
7 Échantillonnage
3
8 Préparation de l'échantillon pour essai
3
9 Mode opératoire
4
9.1 Préparation des solutions d'étalonnage
4
9.2 Optimisation des paramètres de travail
5
9.3 Préparation de la solution d'essai
5
9.4 Détermination
5
10 Expression des résultats
6
11 Fidélité
7
11.1 Essai interlaboratoire
7
11.2 Répétabilité
7
11.3 Reproductibilité
7
12 Rapport d'essai
7
Annexe A (informative) Exemple de chromatogrammes
8
Annexe B (informative) Saponification
10
Annexe C (informative) Résultats des essais interlaboratoires
12
Bibliographie
17

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