NF ISO 10381-6
Soil quality - Sampling - Part 6 : guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
Le présent document propose des lignes directrices relatives à la collecte, la manipulation et la conservation de sols destinés principalement à l'étude en laboratoire de l'activité microbienne aérobie.Il décrit comment réduire les conséquences des différences de température, de la teneur en eau et en oxygène disponible sur les processus aérobies afin d'obtenir des mesures reproductibles en laboratoire.Il s'applique principalement aux sols tempérés. Les sols collectés de lieux où les climats sont extrêmes (par exemple le pergélisol, les sols tropicaux) peuvent nécessiter une manipulation particulière.
Le présent document propose des lignes directrices relatives à la collecte, la manipulation et la conservation de sols destinés principalement à l'étude en laboratoire de l'activité microbienne aérobie. Il décrit comment réduire les conséquences des différences de température, de la teneur en eau et en oxygène disponible sur les processus aérobies afin d'obtenir des mesures reproductibles en laboratoire. Il s'applique principalement aux sols tempérés. Les sols collectés de lieux où les climats sont extrêmes (par exemple le pergélisol, les sols tropicaux) peuvent nécessiter une manipulation particulière.
ISO 18400-102:2017 gives guidelines for techniques for taking samples so that these can subsequently be examined for the purpose of providing information on soil quality. It gives information on equipment that is typically applicable in particular sampling situations to enable correct sampling procedures to be carried out and representative samples to be collected. Guidance is given on the selection of the equipment and the techniques to use to enable both disturbed and undisturbed samples to be correctly taken at different depths. ISO 18400-102:2017 does not cover: - investigations for geotechnical purposes, though where redevelopment of a site is envisaged, the soil quality investigation and the geotechnical investigation may sometimes be beneficially combined; - sampling of hard strata such as bedrock; - methods for the collection of information on soil quality without taking samples such as geophysical methods; - collection of water samples (these are to be collected in accordance with appropriate International Standards on ground or surface water sampling; for further information, see the ISO 5667 series); - investigations of soil gas about which guidance is provided in ISO 18400‑204; - investigation of radioactively contaminated sites. NOTE 1 "Sampling technique" is defined in ISO 11074. NOTE 2 Guidance on the investigation and assessment of radioactivity in soils is provided in the ISO 18589 series.
ISO 18400-105:2017 establishes general principles for packing, preservation, transport and delivery of samples of soil and related materials with an emphasis on requirements for when chemical analysis of the samples is required, but with the intention that the general procedures are to be adapted as appropriate when other forms of testing are required (e.g. biological testing, physical tests on disturbed or undisturbed samples). Special procedures for specific sampling purposes are given in other parts of ISO 18400 (see also 7.2). ISO 18400-105:2017 is intended to be read in conjunction with ISO 18512.
This document gives general guidance on the development of site investigation strategies and detailed guidance on the development of sampling strategies, when collecting information on — the average properties of soil, — the variability of soil properties, and — the spatial distribution of soil properties. It is applicable to soil samples intended for chemical testing and determination of a variety of other properties (e.g. physical). Although the main focus of this document is the collection of material (field samples) for transfer to a laboratory for testing, it is also applicable when measurements are made directly in the field. NOTE 1 This document also provides information on the statistical principles underlying the development of appropriate sampling strategies and statistical methodologies. NOTE 2 Guidance on other forms of related sampling activities are given in other International Standards [for soil gas (ISO 18400-204) and for biological testing purposes (ISO 18400-206)]. Guidance on sampling groundwater is provided in ISO 5667-11 and ISO 5667-22 and on sampling methods and groundwater measurements in geotechnical investigations in ISO 22475-1.
This document provides standard procedures for the collection, handling and storage of soil for subsequent biological testing under aerobic conditions in the laboratory. It applies to the collection, handling and storage for assessing the effects of soil on microorganisms, invertebrates (e.g. survival, reproduction, growth, behaviour) and plants (e.g. development, growth). This document is not applicable to the handling of soil where anaerobic conditions need to be maintained throughout. This document describes how to minimize the effects of differences in temperature, water content, and availability of oxygen on aerobic processes as well as the fractionation of soil particles to facilitate reproducible laboratory determinations[1][2]. This document is mainly applicable to temperate soils. Soils collected from extreme climates (e.g. permafrost, tropical soils) can require special handling. NOTE This document does not provide standard procedures on the collection, handling and storage of soil organisms when assessing the structure and function of soil organism communities in the field. Such standard procedures are provided in ISO 23611‑1 to ISO 23611‑6.
- Avant-proposiv
- Introductionv
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1 Domaine d'application1
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2 Termes et définitions1
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3 Mode opératoire1
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3.1 Choix des sites d'échantillonnage1
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3.2 Description du lieu de prélèvement2
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3.3 Conditions d'échantillonnage2
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3.4 Méthodes d'échantillonnage2
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3.5 Étiquetage des échantillons2
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3.6 Conditions de transport2
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3.7 Traitement du sol2
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3.8 Conditions et durées de conservation3
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3.9 Pré-incubation4
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4 Rapport d'essai4
- Bibliographie6
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