NF ISO 17601

NF ISO 17601

April 2016
Standard Cancelled

Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups. It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

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Main informations

Collections

National standards and national normative documents

Publication date

April 2016

Number of pages

37 p.

Reference

NF ISO 17601

ICS Codes

13.080.30   Biological properties of soils

Classification index

X31-235

Print number

1

International kinship

ISO 17601:2016
Sumary
Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

Standard replaced by (1)
NF EN ISO 17601
June 2018
Standard Current
Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

<p>ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.</p> <p>It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.</p>

Table of contents
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  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Matériel d'essai
  • 6 Appareillage
  • 7 Mode opératoire
  • 8 Examen des étapes critiques de l'essai de qPCR
  • 9 Expression des résultats de l'essai de qPCR
  • 10 Étude interlaboratoires internationale
  • 11 Rapport d'essai
  • Annexe A Description des principales étapes d'un essai de qPCR TaqMan
  • Annexe B Étude interlaboratoires internationale relative à l'évaluation de la qPCR pour quantifier l'abondance de séquences spécifiques de gènes microbiens à partir d'ADN extrait directement du sol
  • Bibliographie
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