XP ISO/TS 21872-1

June 2007

Standard Cancelled

Microbiology of food and animal feeding stuffs - Horizontal method for the detection of potentially enteropathogenic Vibrio spp. - Part 1 : detection of Vibrio parahaemolyticus and Vibrio cholerae

ISO/TS 21872-1:2007 specifies a horizontal method for the detection of the two main pathogenic Vibrio species causing intestinal illness in humans: V. parahaemolyticus and V. cholerae.It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

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Main informations

Collections

National standards and national normative documents

Publication date

June 2007

Number of pages

27 p.

Reference

XP ISO/TS 21872-1

ICS Codes

07.100.30 Food microbiology

Classification index

V08-064-1

Print number

1 - 18/07/2007

International kinship

ISO/TS 21872-1:2007

Sumary

Microbiology of food and animal feeding stuffs - Horizontal method for the detection of potentially enteropathogenic Vibrio spp. - Part 1 : detection of Vibrio parahaemolyticus and Vibrio cholerae

ISO/TS 21872-1:2007 specifies a horizontal method for the detection of the two main pathogenic Vibrio species causing intestinal illness in humans: V. parahaemolyticus and V. cholerae.

It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

Standard replaced by (1)

NF EN ISO 21872-1

September 2017

Standard Current

Microbiology of the food chain - Horizontal method for the determination of Vibrio spp - Part 1 : detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus

ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. ISO 21872-1:2017 is applicable to the following: - products intended for human consumption and the feeding of animals; - environmental samples in the area of food production and food handling.

Table of contents

Avant-propos
iv
Introduction
v
1 Domaine d'application
1
2 Références normatives
1
3 Termes et définitions
2
4 Principe
2
4.1 Généralités
2
4.2 Premier enrichissement en milieu sélectif liquide
2
4.3 Second enrichissement en milieu sélectif liquide
2
4.4 Isolement et identification
2
4.5 Confirmation
2
5 Milieux de culture et réactifs
3
5.1 Milieu d'enrichissement: Eau peptonée alcaline salée (EPAS)
3
5.2 Milieux d'isolement sélectifs solides
3
5.3 Gélose nutritive salée (GNS)
3
5.4 Réactif pour la recherche de l'oxydase
3
5.5 Gélose salée aux trois sucres et au fer (TSI)
3
5.6 Milieu salé pour la détection de l'ornithine décarboxylase (ODC)
3
5.7 Milieu salé pour la détection de la lysine décarboxylase (LDC)
3
5.8 Milieu salé pour la détection de l'arginine dihydrolase (ADH)
3
5.9 Réactif pour la recherche de la v-galactosidase
3
5.10 Milieu salé pour la recherche de l'indole
4
5.11 Eaux peptonées salées
4
5.12 Solution de chlorure de sodium
4
6 Appareillage et verrerie
4
7 Échantillonnage
4
8 Préparation de l'échantillon pour essai
4
9 Mode opératoire (voir Annexe A)
4
9.1 Prise d'essai et suspension mère
4
9.2 Premier enrichissement sélectif
5
9.3 Second enrichissement sélectif
5
9.4 Isolement et identification
5
9.5 Confirmation
6
10 Expression des résultats
10
11 Rapport d'essai
10
Annexe A (normative) Schéma du mode opératoire
11
Annexe B (normative) Composition et préparation des milieux de culture et des réactifs
12
Bibliographie
19

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