NF EN ISO 20976-1

NF EN ISO 20976-1

April 2019
Standard Current

Microbiology of the food chain - Requirements and guidelines for conducting challenge tests of food and feed products - Part 1 : challenge tests to study growth potential, lag time and maximum growth rate

This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products. The use of this document can be extended to yeasts that do not form mycelium.

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Main informations

Collections

National standards and national normative documents

Publication date

April 2019

Number of pages

38 p.

Reference

NF EN ISO 20976-1

ICS Codes

07.100.30   Food microbiology

Classification index

V01-009-1

Print number

1

International kinship

ISO 20976-1:2019

European kinship

EN ISO 20976-1:2019
Sumary
Microbiology of the food chain - Requirements and guidelines for conducting challenge tests of food and feed products - Part 1 : challenge tests to study growth potential, lag time and maximum growth rate

This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.

The use of this document can be extended to yeasts that do not form mycelium.

Replaced standards (1)
NF V01-009
May 2014
Standard Cancelled
Food traceability and safety - Management and hygiene - Guidance for performing microbiological growth tests

Le présent document décrit les protocoles de réalisation de tests de croissance pour l'étude du comportement de bactéries végétatives ou sporulées dans une matrice alimentaire soit sur les produits finis, soit sur les produits en cours d'élaboration.

Table of contents
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  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Appareillage
  • 6 Milieux de culture et réactifs
  • 7 Plan de l'étude et échantillonnage
  • 8 Choix des souches
  • 9 Préparation de l'inoculum
  • 10 Inoculation des unités pour essai
  • 11 Témoins
  • 12 Conservation des unités pour essai
  • 13 Analyse
  • 14 Expression des résultats
  • 15 Rapport d'essai
  • Annexe A Évaluation de la variabilité inter-lots prenant en compte le pH et l'aw
  • Annexe B Nombre minimum d'unités à préparer pour l'étude du test de croissance
  • Annexe C Exemples de protocoles de préparation des inocula
  • Annexe D Exemples de procédures permettant d'estimer le potentiel de croissance, le temps de latence et le taux de croissance maximal à partir des tests de croissance
  • Annexe E Utilisation de la simulation pour évaluer une population microbienne pour différentes conditions de température
  • Bibliographie
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